MicroRNA-9a ensures the precise specification of sensory organ precursors in Drosophila
MicroRNA-9a ensures the precise specification of sensory organ precursors in Drosophila
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DOI：
10.1101/gad.1466306
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发表时间：
2006-10-15
影响因子：
10.5
通讯作者：
Gao, Fen-Biao
中科院分区：
文献类型：
--
作者：
Li, Yan;Wang, Fay;Gao, Fen-Biao
MicroRNAs(MiRNAs)参与了动物发育的各个方面的调控，但其在神经发生中的作用尚不清楚。在这里，我们报道了果蝇周围神经系统miR-9a功能的丧失导致感觉器官前体(SOP)的异位产生，而miR-9a的过度表达导致SOP的严重丧失。我们进一步证明了miR-9a和无感觉(SENS)之间的强烈遗传相互作用，控制了成体翅膀想象盘中SOP的形成。此外，miR-9a通过其3‘非翻译区抑制SENS的表达。MiR-9a在上皮细胞中表达，包括那些与神经丛内SOP相邻的细胞，这表明miR-9a通常通过下调SENS的表达来抑制非SOP细胞中的神经元命运。这些结果表明，miR-9a确保在发育过程中产生准确数量的神经元前体细胞。
MicroRNAs (miRNAs) have been implicated in regulating various aspects of animal development, but their functions in neurogenesis are largely unknown. Here we report that loss of miR-9a function in the Drosophila peripheral nervous system leads to ectopic production of sensory organ precursors (SOPs), whereas overexpression of miR-9a results in a severe loss of SOPs. We further demonstrate a strong genetic interaction between miR-9a and senseless (sens) in controlling the formation of SOPs in the adult wing imaginal disc. Moreover, miR-9a suppresses Sens expression through its 3' untranslated region. miR-9a is expressed in epithelial cells, including those adjacent to SOPs within proneural clusters, suggesting that miR-9a normally inhibits neuronal fate in non-SOP cells by down-regulating Sens expression. These results indicate that miR-9a ensures the generation of the precise number of neuronal precursor cells during development.