Lactobacillus casei prevents impaired barrier function in intestinal epithelial cells
Lactobacillus casei prevents impaired barrier function in intestinal epithelial cells
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DOI：
10.1111/j.1600-0463.2010.02691.x
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发表时间：
2011-01-01
期刊：
影响因子：
2.8
通讯作者：
Park, Yoon Kyung
中科院分区：
文献类型：
--
作者：
Eun, Chang Soo;Kim, Yong Seok;Park, Yoon Kyung
益生菌对上皮屏障功能的确切影响尚未完全了解。本研究的目的是评估尼古丁诱导的肠上皮细胞（IEC）上皮屏障功能障碍，并研究益生菌在预防上皮屏障功能障碍中的作用。用肿瘤坏死因子（TNF）-α或干扰素（IFN）-γ基底外侧刺激在transwell小室上生长的Caco-2细胞。益生菌，干酪乳杆菌，加入细胞因子刺激前1小时。在L.干酪素刺激。测量Caco-2单层的电阻和细胞旁通透性。通过免疫荧光评估闭合小带（ZO）-1蛋白的分布，并进行ZO-1、p-Akt和toll样受体（TLR）2的Western印迹分析。TNF-α和IFN-γ对Caco-2细胞的刺激均降低了跨上皮阻力（TER），增加了上皮通透性，并降低了Caco-2细胞的ZO-1表达。相比之下，L. casei逆转了酪氨酸诱导的TER、上皮通透性和ZO-1表达的功能障碍。L.对精氨酸诱导的TER和肠通透性功能障碍的拮抗作用。用MAPK抑制剂处理消除了casei。干酪乳杆菌刺激Caco-2细胞可增加TLR 2和p-Akt表达。益生菌湖酪蛋白，防止肠上皮细胞中的精氨酸诱导的上皮屏障功能障碍。
The exact effect of probiotics on epithelial barrier function is not well understood. The aims of this study were to evaluate cytokine-induced epithelial barrier dysfunction in intestinal epithelial cells (IECs) and to study the role of probiotics in the prevention of epithelial barrier dysfunction. Caco-2 cells grown on transwell chambers were stimulated with tumor necrosis factor (TNF)-alpha or interferon (IFN)-gamma basolaterally. Probiotic, Lactobacillus casei, was added 1 h before cytokine stimulation. MAPK inhibitors were added 15 min before L. casei stimulation. The electrical resistance and paracellular permeability of Caco-2 monolayers were measured. Distribution of zonula occludens (ZO)-1 protein was assessed by immunofluorescence, and Western blot analyses for ZO-1, p-Akt, and toll-like receptor (TLR) 2 were performed. Both TNF-alpha and IFN-gamma stimulation on Caco-2 cells decreased transepithelial resistance (TER), increased epithelial permeability, and decreased ZO-1 expression of Caco-2 cells. In contrast, pretreatment of L. casei reversed the cytokine-induced dysfunction of TER, epithelial permeability, and ZO-1 expression. Reversal of cytokine-induced dysfunction of TER and intestinal permeability by L. casei was abrogated with MAPK inhibitor treatment. Lactobacillus casei stimulation on Caco-2 cells increased TLR2 and p-Akt expression. Probiotic, L. casei, prevents cytokine-induced epithelial barrier dysfunctions in IECs.