A large-tumor-antigen-specific monoclonal antibody inhibits DNA replication of simian virus 40 minichromosomes in an in vitro elongation system
A large-tumor-antigen-specific monoclonal antibody inhibits DNA replication of simian virus 40 minichromosomes in an in vitro elongation system
复制标题
大肿瘤抗原特异性单克隆抗体在体外延伸系统中抑制猿猴病毒 40 微型染色体的 DNA 复制
作者：
H. Stahl;P. Dröge;H. Zentgraf;R. Knippers
In productively infected cells, a fraction of large-tumor antigen (T antigen) is tightly bound to replicating simian virus 40 (SV40) minichromosomes and does not dissociate at salt concentrations of greater than 1 M NaCl. We present electronmicrograms demonstrating the presence of T antigen on the replicated sections of replicating SV40 minichromosomes. We also show that the fraction of tightly bound T antigen is recognized by antibodies from mouse tumor serum and, more specifically, by a particular T-antigen-specific monoclonal antibody, PAb 1630. A second T-antigen-specific monoclonal antibody, PAb 101, does not react with the T-antigen fraction remaining on replicating SV40 chromatin at high salt concentrations. We used an in vitro replication system which allows, via semiconservative DNA replication, the completion of in vivo-initiated replicative intermediate DNA molecules. We show that monoclonal antibody PAb 1630, but not monoclonal antibody PAb 101, inhibits viral DNA replication. We discuss the possibility that SV40 T antigen may play a role in chain elongation during SV40 chromatin replication.
SV40 A 蛋白与 DNA 的另一种相互作用。
影响因子：
3.7
作者：
Tegtmeyer,P;Andersen,B;Shaw,SB;Wilson,VG
通讯作者：
Wilson,VG
使用单克隆抗体作为猿猴病毒40 T抗原ATP酶活性的探针。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Clark,R;Lane,DP;Tjian,R
通讯作者：
Tjian,R
影响因子：
--
作者：
Levine,AJ
通讯作者：
Levine,AJ