Structure of the cytoplasmic β subunit -: T1 assembly of voltage-dependent K+ channels
Structure of the cytoplasmic β subunit -: T1 assembly of voltage-dependent K+ channels
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DOI：
10.1126/science.289.5476.123
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发表时间：
2000-07-07
期刊：
影响因子：
56.9
通讯作者：
MacKinnon, R
中科院分区：
文献类型：
--
作者：
Gulbis, JM;Zhou, M;MacKinnon, R
电压依赖性K+通道的细胞质组装结构通过X射线晶体学以2.1埃分辨率解决。该组装体包括完整膜或亚基的胞质（T1）结构域以及氧化还原酶β亚基，形成四重对称T1（4）β（4）复合物。电生理学分析表明，该复合物的取向为四个T1结构域面向跨膜孔，四个β亚基面向细胞质。跨膜孔通过T1（4）β（4）复合物上方的带负电荷的侧向开口与细胞质连通。电压依赖性K+通道的失活肽通过进入这些开口到达其作用位点。
The structure of the cytoplasmic assembly of voltage-dependent K+ channels was solved by x-ray crystallography at 2.1 angstrom resolution. The assembly includes the cytoplasmic (T1) domain of the integral membrane or subunit together with the oxidoreductase beta subunit in a fourfold symmetric T1(4)beta(4) complex. An electrophysiological assay showed that this complex is oriented with four T1 domains facing the transmembrane pore and four beta subunits facing the cytoplasm. The transmembrane pore communicates with the cytoplasm through lateral, negatively charged openings above the T1(4)beta(4) complex. The inactivation peptides of voltage-dependent K+ channels reach their site of action by entering these openings.