Rohon-Beard sensory neurons are induced by BMP4 expressing non-neural ectoderm in Xenopus laevis
Rohon-Beard sensory neurons are induced by BMP4 expressing non-neural ectoderm in Xenopus laevis
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DOI：
10.1016/j.ydbio.2007.11.036
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发表时间：
2008-02-15
影响因子：
2.7
通讯作者：
Artinger, Kristin Bruk
中科院分区：
文献类型：
--
作者：
Rossi, Christy Cortez;Hernandez-Lagunas, Laura;Artinger, Kristin Bruk
Rohon-Beard机械感觉神经元（RB）、神经嵴细胞和神经源性基板在脊椎动物发育过程中出现于神经外胚层和非神经外胚层的边界。神经嵴细胞需要表达BMP的非神经外胚层来诱导。为了确定表皮外胚层衍生的BMP信号传导是否也参与RB感觉神经元的诱导，将来自供体非洲爪蟾胚胎的神经板的内侧区域移植到处于相同发育阶段的宿主胚胎的非神经腹侧外胚层中。在移植部位诱导神经板边缘和RB，如分别通过Xblimp 1和AHox 11 L2和XN-tubulin的表达所示。有色供体和白化病宿主之间的移植研究表明，供体神经和宿主表皮组织中的神经元均被诱导。由于需要中等水平的BMP 4信号传导来诱导神经板边缘命运，我们直接测试了BMP 4诱导RB的能力;浸泡在1或10 ng/ml中的珠能够在培养的神经板组织中诱导RB。相反，当来自BMP活性降低的胚胎的神经板组织（来自注射头蛋白或显性负性BMP受体）被移植到未操作宿主的非神经外胚层中时，RB不能形成。我们的结论是神经和非神经外胚层之间的接触是能够诱导RB，BMP 4可以诱导RB标记，和BNIP活性是诱导异位RB感觉神经元所必需的。(c)2007年爱思唯尔公司All rights reserved.
Rohon-Beard mechanosensory neurons (RBs), neural crest cells, and neurogenic placodes arise at the border of the neural- and non-neural ectoderm during anamniote vertebrate development. Neural crest cells require BMP expressing non-neural ectoderm for their induction. To determine if epidermal ectoderm-derived BMP signaling is also involved in the induction of RB sensory neurons, the medial region of the neural plate from donor Xenopus laevis embryos was transplanted into the non-neural ventral ectoderm of host embryos at the same developmental stage. The neural plate border and RBs were induced at the transplant sites, as shown by expression of Xblimp1, and AHox11L2 and XN-tubulin, respectively. Transplantation studies between pigmented donors and albino hosts showed that neurons are induced both in donor neural and host epidermal tissue. Because an intermediate level of BMP4 signaling is required to induce neural plate border fates, we directly tested BMP4s ability to induce RBs; beads soaked in either 1 or 10 ng/ml were able to induce RBs in cultured neural plate tissue. Conversely, RBs fail to form when neural plate tissue from embryos with decreased BMP activity, either from injection of noggin or a dominant negative BMP receptor, was transplanted into the non-neural ectoderm of un-manipulated hosts. We conclude that contact between neural and non-neural ectoderm is capable of inducing RBs, that BMP4 can induce RB markers, and that BNIP activity is required for induction of ectopic RB sensory neurons. (c) 2007 Elsevier Inc. All rights reserved.