REGIONAL AND AGE VARIATIONS IN GROWING TENDON
REGIONAL AND AGE VARIATIONS IN GROWING TENDON
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DOI：
10.1002/jmor.1052210306
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发表时间：
1994-09-01
影响因子：
1.5
通讯作者：
VAILAS, AC
中科院分区：
文献类型：
--
作者：
CURWIN, SL;ROY, RR;VAILAS, AC
将10只6、8和12周龄的白来客那鸡的腓肠肌肌腱分为近端、中端和远端部分，以评估其组成和生长的区域差异。在研究期间，体重增加了150%，而腓肠肌和肌腱分别增加了193%和227%。随着年龄的增长，细胞结构（DNA浓度）或羟基吡啶（OHP）交联没有显著变化。羟脯氨酸（HYP）浓度在12周龄时增加，而己醛酸盐、葡萄糖胺和半乳糖胺则减少。成分显示出一定的区域差异：与近端或中间区域相比，肌腱远端区域的HYP浓度较低，GAGs和OHP交联增加，两者之间没有差异。胶原原纤维的平均直径随着年龄的增长而增加，但最老的肌腱也含有更多的小直径原纤维（< 40 nm）。在所有三个年龄都有单峰型的原纤维分布，尽管这种分布在12周时扩大了。本研究的数据表明，在整个研究期间，肌腱都在快速生长，而成熟肌腱的特征变化，如OHP交联浓度的增加，由于大量新组织的产生，尚未在孵化中发展。虽然肌腱的所有三个区域在大小上相似，但远端区域的组成不同于近端和中间区域，这表明在采集肌腱时应避免这部分肌腱。(C) 1994 Wiley-Liss, Inc。
Gastrocnemius tendons of 10 White Leghorn chickens at 6, 8, and 12 weeks of age were divided into proximal, middle, and distal portions to assess regional variability in composition and growth. Body weight increases similar to 150% during the period examined, whereas the lateral gastrocnemius muscle and tendon increase similar to 193% and 227%, respectively. No significant changes in cellularity (DNA concentration) or hydroxypyridinium (OHP) crosslinks occur with increasing age. Hydroxyproline (HYP) concentration increases by 12 weeks of age, as hexuronate, glucosamine, and galactosamine decrease. Composition shows some regional variation: the distal region of the tendon has a lower HYP concentration and increased GAGs and OHP crosslinks compared to either the proximal or middle regions, which do not differ from each other. The mean collagen fibril diameter increases with age, but the oldest tendons also contain more small diameter fibrils (< 40 nm). There is a unimodal fibril distribution at all three ages, although this has broadened by 12 weeks. The data from this study suggest that rapid tendon growth occurs throughout the time period examined and that changes characteristic of mature tendon, such as increased OHP crosslink concentration, have not yet developed in hatchlings because of the large amount of new tissue being produced. Whereas all three regions of the tendon are similar in size, composition of the distal region differs from that of the proximal and middle regions, suggesting that this portion of the tendon should be avoided when sampling a tendon. (C) 1994 Wiley-Liss, Inc.