Cloning high molecular weight DNA fragments by the bacteriophage P1 system.
Cloning high molecular weight DNA fragments by the bacteriophage P1 system.
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通过噬菌体 P1 系统克隆高分子量 DNA 片段。
DOI：
10.1016/0168-9525(92)90018-y
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
N. Sternberg
中科院分区：
文献类型：
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作者：
N. Sternberg
用于高分子量基因组DNA片段的P1 donirLg的两个载体：(a)pNS582tet14Ad10(pAd10)：(b)pAd10sacBI1。文中描述了载体的所有元素及其在克隆中的用途。限制位点：Bam、BamHI； Sal，SalI：Sca，ScaI：Sfi，SfiI：Not，Notl。还显示了pAd10sacBII载体中Sp6和T7启动子以及P1 cl阻遏物结合位点（大'T'形）的位置和方向。
The two vectors used for P1 donirLg of high molecular weight genomic DNA fragments:(a) pNS582tet14AdlO (pAdlO):(b) pAdlOsacBIl. All the elements of the vectors and their use in cloning are described in the text. Restriction sites: Bam, BamHI; Sal, SalI: Sca, ScaI: Sfi, SfiI: Not, Notl. Also shown are the locations and directions of Sp6 and T7 promoters and the P1 cl repressor-binding site (the large'T'shape) in the pAdlOsacBII vector.