Immune complex transfer enzyme immunoassay for (anti‐human t‐cell leukemia virus type i) igg in serum using a synthetic peptide, env gp46(188–209), as antigen
Immune complex transfer enzyme immunoassay for (anti‐human t‐cell leukemia virus type i) igg in serum using a synthetic peptide, env gp46(188–209), as antigen
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使用合成肽 env gp46(188-209) 作为抗原，对血清中的（抗人 T 细胞白血病病毒 i 型）igg 进行免疫复合物转移酶免疫测定
DOI：
10.1002/jcla.1860050106
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发表时间：
1991
影响因子：
2.7
通讯作者：
I. Sakoda
中科院分区：
文献类型：
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作者：
T. Kohno;E. Ishikawa;I. Sakoda
描述了一种使用化学安全合成肽env gp 46（188-209）测定血清中（抗人T细胞白血病病毒I型）IgG（抗HTLV I IgG）的免疫复合物转移酶免疫测定法。与过量无活性β-D-半乳糖苷酶孵育以消除抗-β-D-半乳糖苷酶抗体干扰的试验血清中的抗-HTLV-I IgG，同时与二硝基苯基牛血清白蛋白-env gp 46（188-209）-β-D-半乳糖苷酶结合物反应。将由三种组分形成的复合物捕获到涂有亲和纯化（抗二硝基苯基）IgG的聚苯乙烯球上。清洗以消除试验血清中的非特异性IgG和过量的β-D-半乳糖苷酶结合物后，用二硝基苯-L-赖氨酸从聚苯乙烯球上洗脱复合物，并转移到包被有亲和纯化（抗人IgG γ链）IgG的聚苯乙烯球上。通过荧光测定法测定与（抗人IgG γ链）IgG包被的聚苯乙烯球结合的β-D-半乳糖苷酶活性。该检测试剂盒灵敏度高，可检出常规酶免疫分析和Western印迹法阴性的血清样本中的抗HTLV-I IgG。用过量的env gp 46（188-209）预孵育试验血清，证实了该试验的特异性。然而，也注意到一些不利之处。
An immune complex transfer enzyme immunoassay for (anti‐human T‐cell leukemia virus type I) IgG (anti‐HTLV‐I IgG) in serum using a chemically and safely synthesized peptide, env gp46(188–209), is described. Anti‐HTLV‐I IgG in test serum, which had been incubated with excess of inactive β‐D‐galactosidase to eliminate interference by anti‐β‐D‐galactosidase antibodies, was reacted simultaneously with dinitrophenyl bovine serum albumin‐env gp46(188–209)‐β‐D‐galactosidase conjugate. The complex formed of the three components was trapped onto polystyrene balls coated with affinity‐purified (anti‐dinitrophenyl group) IgG. After washing to eliminate nonspecific IgG in the test serum and excess of the β‐D‐galactosidase conjugate, the complex was eluted from the polystyrene balls with dinitrophenyl‐L‐lysine and transferred to polystyrene balls coated with affinity‐purified (anti‐human IgG γ‐chain) IgG. β‐D‐Galactosidase activity bound to the (anti‐human IgG γ‐chain) IgG‐coated polystyrene balls was assayed by fluorometry. This assay was sensitive and detected anti‐HTLV‐I IgG in serum samples which were negative by the conventional enzyme immunoassay and Western blotting. And the specificity of this assay was confirmed by preincubation of test serum with excess of env gp46(188–209). However, some disadvantages were also noted.
使用 env 编码的合成肽和 gp46 单克隆抗体绘制人 T 细胞白血病病毒 I 型 (HTLV-I) gp46 和 gp21 包膜糖蛋白的免疫原性区域。
DOI：
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发表时间：
1989
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Palker,TJ;Tanner,ME;Scearce,RM;Streilein,RD;Clark,ME;Haynes,BF
通讯作者：
Haynes,BF