Three-dimensional modeling of angiogenesis in porous biomaterial scaffolds
Three-dimensional modeling of angiogenesis in porous biomaterial scaffolds
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DOI：
10.1016/j.biomaterials.2012.12.047
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发表时间：
2013-04-01
期刊：
影响因子：
14
通讯作者：
Cinar, Ali
中科院分区：
文献类型：
--
作者：
Mehdizadeh, Hamidreza;Sumo, Sami;Cinar, Ali
生物材料支架的血管化是工程化组织成功应用于临床的关键。实验研究经常被用来研究支架结构对血管化组织形成的作用。然而，实验是昂贵的和耗时的，并且合成方案通常不允许对特定支架特性的独立研究。计算模型允许快速筛选潜在的材料设计，控制支架性能，这在实验室环境中是困难的。我们已经开发和测试了一个三维的代理为基础的框架，研究支架孔结构对血管生成的影响。软件代理代表内皮细胞，它们相互作用并与它们的微环境相互作用，导致血管侵入支架。由实验结果驱动的规则库控制着个体代理的行为。模拟具有明确定义的均质和非均质孔结构的3D支架模型，以研究各种设计参数的影响。模拟结果表明，具有较高互连性和孔隙率的较大尺寸的孔支持快速和广泛的血管生成。开发的框架可用于筛选生物材料支架设计，以实现最佳血管化，并研究入侵血管及其微环境之间的复杂相互作用。(C)2013爱思唯尔有限公司保留所有权利。
Vascularization of biomaterial scaffolds is essential for the successful clinical application of engineered tissues. Experimental studies are often performed to investigate the role of scaffold architecture on vascularized tissue formation. However, experiments are expensive and time-consuming and synthesis protocols often do not allow for independent investigation of specific scaffold properties. Computational models allow for rapid screening of potential material designs with control over scaffold properties that is difficult in laboratory settings. We have developed and tested a three-dimensional agent-based framework for investigating the effect of scaffold pore architecture on angiogenesis. Software agents represent endothelial cells, interacting together and with their micro-environment, leading to the invasion of blood vessels into the scaffold. A rule base, driven by experimental findings, governs the behavior of individual agents. 3D scaffold models with well-defined homogeneous and heterogeneous pore architectures were simulated to investigate the impact of various design parameters. Simulation results indicate that pores of larger size with higher interconnectivity and porosity support rapid and extensive angiogenesis. The developed framework can be used to screen biomaterial scaffold designs for optimal vascularization and investigate complex interactions among invading blood vessels and their micro-environment. (C) 2013 Elsevier Ltd. All rights reserved.