Identification of genes (SPON2 and C20orf2) differentially expressed between cancerous and noncancerous lung cells by mRNA differential display
Identification of genes (SPON2 and C20orf2) differentially expressed between cancerous and noncancerous lung cells by mRNA differential display
复制标题
DOI：
10.1006/geno.1999.5939
复制
发表时间：
1999-10-01
期刊：
影响因子：
4.4
通讯作者：
Yokota，J
中科院分区：
文献类型：
--
作者：
Manda，R;Kohno，T;Yokota，J
应用mRNA差异显示技术对3株小细胞肺癌（SCLC）细胞系、6株非小细胞肺癌（NSCLC）细胞系和3例正常肺组织进行分析，筛选肺癌细胞与正常肺组织、SCLC细胞与NSCLC细胞之间差异表达的基因。我们分离出五个差异表达的基因，其中两个是新的，三个是已知的。DIL-1（在癌性和非癌性肺细胞中差异表达; HGMW批准的符号SPON 2）和肺表面活性物质脱辅基蛋白A在正常肺组织中表达，但在肺癌细胞系中不表达，而DIL-2（HGMW批准的符号C20 orf 2）和nm 23-H1在肺癌细胞系中表达，但在正常肺组织中不表达。剩余的基因，膜联蛋白II，在小细胞肺癌中的表达水平低于非小细胞肺癌和正常肺组织。这些基因在原发性肺癌中也有差异表达。其中一个新基因DIL-1编码一种与Mindin/F-spondin家族同源的分泌蛋白。另一个是DIL-2，编码一个具有ATP/GTP结合位点基序的蛋白。这些数据提供了必要的基本信息，以了解肺癌和正常肺，小细胞肺癌和NSCLC之间的基因表达谱的差异。这些基因的进一步鉴定将有助于阐明人类肺癌发生的分子机制。
mRNA differential display was applied to three small cell lung carcinoma (SCLC) cell lines, six non-small cell lung carcinoma (NSCLC) cell lines, and three normal lung tissues to identify genes differentially expressed between lung carcinoma cells and normal lung tissues and between SCLC cells and NSCLC cells. We isolated five differentially expressed genes, two that were novel and three that were already known. DIL-1 (differentially expressed in cancerous and noncancerous lung cells; HGMW-approved symbol SPON2) and pulmonary surfactant apoprotein A were expressed in normal lung tissues but not in lung carcinoma cell lines, whereas DIL-2 (HGMW-approved symbol C20orf2) and nm23-H1 were expressed in lung carcinoma cell lines but not in normal lung tissues. The remaining gene, Annexin II, was expressed at a lower level in SCLC than in NSCLC and normal lung tissues. These genes were also differentially expressed in primary lung cancers. One of the two novel genes, DIL-1, encodes a secreted protein homologous to the Mindin/F-spondin family. The other, DIL-2, encodes a protein with a putative ATP/GTP binding site motif. These data provide basic information necessary to understand the differences in gene expression profiles between lung carcinoma and normal lung and between SCLC and NSCLC. Further characterization of these genes will help to clarify the molecular mechanisms of human lung carcinogenesis.