Inflammation elevated IL-33 originating from the lung mediates inflammation in acute lung injury
Inflammation elevated IL-33 originating from the lung mediates inflammation in acute lung injury
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源自肺部的炎症升高的 IL-33 介导急性肺损伤中的炎症
DOI：
10.1016/j.clim.2016.10.014
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发表时间：
2016-12-01
影响因子：
8.6
通讯作者：
Xu, Fang
中科院分区：
文献类型：
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作者：
Lin, Shi-hui;Fu, Juan;Xu, Fang
急性呼吸窘迫综合征（ARDS）是一种严重的炎症反应，其发病机制尚不清楚。在这里，我们调查了白细胞介素（IL）-33是否在ARDS患者中升高。从14名ARDS患者和24名对照健康志愿者获得血清样品。ELISA法测定IL-33的浓度。另外，我们建立了小鼠肺内和肺外ARDS模型，并采集血清和肺组织标本进行分析。结果显示，肺部ARDS患者血清IL-33浓度明显高于对照组。此外，IFN-γ和IL-2水平与IL-33水平呈正相关。我们还发现，在肺ARDS模型中，血清和肺中的IL-33水平均升高。然而，在肺外ARDS模型中情况并非如此。肺ARDS模型中的肺部炎症和损伤通过IL-33中和抗体治疗而减轻。(C)2016 Elsevier Inc. All rights reserved.
Excessive inflammatory reactions occur with acute respiratory distress syndrome (ARDS), however, the underlying mechanisms of ARDS remain incompletely understood. Here we investigated whether interleukin (IL)-33 was elevated in ARDS patients. Serum samples were obtained from 14 ARDS patients and 24 control healthy volunteers. ELISA was used to measure the concentrations of IL-33. Besides, we established pulmonary ARDS and extrapulmonary ARDS models in mice, and serum and lung tissue samples were collected for analyses. The results showed that serum IL-33 concentrations were significantly higher in pulmonary ARDS patients compared to controls. Also, the levels of IFN-gamma and IL-2 were positively correlated with IL-33 levels. We also showed that there were increased IL-33 levels in both the serum and lungs in the pulmonary ARDS model. This was not the case, however, in the extrapulmonary ARDS model. Pulmonary inflammation and injury in the pulmonary ARDS model was reduced with IL-33 neutralizing antibody treatment. (C) 2016 Elsevier Inc. All rights reserved.