Malondialdehyde content as a potential biochemical indicator of tolerant Cenchrus ciliaris L. genotypes under heat stress treatment
Malondialdehyde content as a potential biochemical indicator of tolerant Cenchrus ciliaris L. genotypes under heat stress treatment
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DOI：
10.1111/j.1365-2494.2012.00851.x
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发表时间：
2012-09
影响因子：
2.4
通讯作者：
E. Tommasino;S. Griffa;K. Grunberg;A. Ribotta;E. L. Colomba;E. Carloni;M. Quiroga;C. Luna
中科院分区：
文献类型：
--
作者：
E. Tommasino;S. Griffa;K. Grunberg;A. Ribotta;E. L. Colomba;E. Carloni;M. Quiroga;C. Luna
牛尾草（Cenchrus ciliaris L.）是干旱、半干旱地区重要的牧草。作为遗传改良计划的一部分，在温室实验中通过对热胁迫的耐受性将四种基因型[Biloela（BI）、Americana（Am）、Texas（Tx）和Sexual（Sx）]分类。在播种后30天，将一半的植物（对照植物）转移到生长室（28 ℃昼夜），另一半（预处理植物，Prt）暴露于热胁迫处理（0，24，48和72小时; 45 ℃昼夜）。丙二醛（MDA）含量，氧化损伤的指标，从叶片样品测定。在热胁迫过程中，Sx表现出最早的MDA增加（在24 h），其次是Tx（48 h）和Am和BI（72 h）。结果进行了比较，热胁迫耐受性评价的形态特征在休养生息结束时（播种后60天）。鲜重和气生株高在Prt-Sx基因型中最低，在Am和Bl基因型中最高; Tx表现出中等耐性。结果表明，热胁迫下，C。ciliaris基因型可能与调节氧化损伤增加的能力有关。因此，叶片MDA含量可能被用来在一个遗传改良计划的C。因此，纤毛菌作为一种潜在的生化指标，可用于快速、简便、低成本地鉴定耐热基因型。
Buffel grass (Cenchrus ciliaris L.) is an important forage grass in arid and semiarid regions. As part of a genetic improvement programme, four genotypes [Biloela (Bl), Americana (Am), Texas (Tx) and Sexual (Sx)] were categorized by tolerance to heat stress in a greenhouse experiment. At 30 d after sowing, half of the plants (control plants) were transferred to growth chambers (28� C day per night), and the other half (pre-treated plants, Prt) were exposed to heat stress treatment (0, 24, 48 and 72 h; 45� C day per night). Malondialdehyde (MDA) content, an indicator of oxidative damage, was determined from foliar samples. During heat stress, Sx showed the earliest increase in MDA (at 24 h) followed by Tx (48 h) and Am and Bl (72 h). Results were compared with heat-stress tolerance evaluated as morphological traits at the end of recuperation (60 d after sowing). Fresh weight and aerial plant height were lowest in the Prt-Sx genotype and highest in Am and Bl genotypes; Tx showed intermediate tolerance. Results suggest that tolerance to heat stress in C. ciliaris genotypes could be related to the capacity for regulating the oxidative damage increase. Foliar MDA content might therefore be used in a genetic improvement programme of C. ciliaris as a potential biochemical indicator for a rapid, simple and low-cost identification of heat-stress tolerant genotypes.