Antitumor effects of inductive hyperthermia using magnetic ferucarbotran nanoparticles on human lung cancer xenografts in nude mice.
Antitumor effects of inductive hyperthermia using magnetic ferucarbotran nanoparticles on human lung cancer xenografts in nude mice.
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DOI：
10.2147/ott.s42815
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发表时间：
2013
影响因子：
4
通讯作者：
Fujimura M
中科院分区：
文献类型：
--
作者：
Araya T;Kasahara K;Nishikawa S;Kimura H;Sone T;Nagae H;Ikehata Y;Nagano I;Fujimura M
诱导热疗对肺癌的影响尚未得到充分的研究。用于感应热疗的磁性纳米颗粒是定制的，价格昂贵。本研究旨在研究阿魏糖糖素在诱导热疗中的应用，并利用肺癌细胞系阐明阿魏糖糖素诱导热疗是否能促进体内抗肿瘤作用。我们在BALB/c nu/nu裸鼠右大腿皮下注射A549细胞。然后将40只移植了A549的小鼠分为三组。第一组为对照组。第2组和第3组小鼠均在肿瘤内注射糖铁素，第3组小鼠进行交变磁场照射。我们评估了热疗过程中的肿瘤温度、肿瘤生长的时间进程、热疗后肿瘤的组织学表现和不良事件。肿瘤内温度迅速升高，并在交变磁场中保持43°C - 45°C 20分钟。1、2组肿瘤体积呈指数增长，3组肿瘤生长明显受到抑制。未观察到严重的不良事件。3组肿瘤组织学表现以坏死为主。诱导热疗是治疗肺癌的一种有益且有前景的方法。糖铁是进一步发展诱导热疗的新工具。
The effects of inductive hyperthermia on lung cancer have yet to be fully investigated. Magnetic nanoparticles used in inductive hyperthermia are made-to-order and expensive. This study was performed to investigate the use of ferucarbotran in inductive hyperthermia and to clarify whether inductive hyperthermia using ferucarbotran promotes antitumor effects in vivo using a lung cancer cell line. We injected A549 cells subcutaneously into the right thighs of BALB/c nu/nu nude mice. Forty mice with A549 xenografts were then classified into three groups. Group 1 was the control group. All mice in groups 2 and 3 had ferucarbotran injected into their tumors, and mice in group 3 were then subjected to alternating magnetic field irradiation. We evaluated tumor temperature during the hyperthermic procedure, the time course of tumor growth, histologic findings in tumors after hyperthermic treatment, and adverse events. Intratumor temperature rose rapidly and was maintained at 43°C–45°C for 20 minutes in an alternating magnetic field. Tumor volumes in groups 1 and 2 increased exponentially, but tumor growth in group 3 was significantly suppressed. No severe adverse events were observed. Histologic findings for the tumors in group 3 revealed mainly necrosis. Inductive hyperthermia using ferucarbotran is a beneficial and promising approach in the treatment of lung cancer. Ferucarbotran is a novel tool for further development of inductive hyperthermia.