Transcriptome response to nitrogen starvation in rice
Transcriptome response to nitrogen starvation in rice
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DOI：
10.1007/s12038-012-9242-2
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发表时间：
2012-09-01
影响因子：
2.9
通讯作者：
Lian, Xingming
中科院分区：
文献类型：
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作者：
Cai, Hongmei;Lu, Yongen;Lian, Xingming
氮是植物生长发育所必需的一种矿质营养元素。氮素供应不足会引起植物体内广泛的生理生化变化。本研究利用AffysseGeneChip水稻基因组芯片分析了氮饥饿条件下水稻转录组的动态变化。氮饥饿诱导或抑制了3518个基因的转录，占基因组的10.88%。这些变化，大多是短暂的，影响各种细胞代谢途径，包括应激反应，初级和次级代谢，分子运输，调节过程和生物体发育。462个或13.1%的N饥饿转录本在根和地上部中表达相似。通过对水稻和拟南芥的比较分析，共鉴定出73个对N饥饿有反应的直链淀粉类，证明了植物间存在保守的N胁迫耦合机制。对microRNA转录谱的进一步分析揭示了氮饥饿条件下miR399和miR530的差异表达，表明它们在植物营养稳态中的潜在作用。
Nitrogen is an essential mineral nutrient required for plant growth and development. Insufficient nitrogen (N) supply triggers extensive physiological and biochemical changes in plants. In this study, we used Affymetrix GeneChip rice genome arrays to analyse the dynamics of rice transcriptome under N starvation. N starvation induced or suppressed transcription of 3518 genes, representing 10.88% of the genome. These changes, mostly transient, affected various cellular metabolic pathways, including stress response, primary and secondary metabolism, molecular transport, regulatory process and organismal development. 462 or 13.1% transcripts for N starvation expressed similarly in root and shoot. Comparative analysis between rice and Arabidopsis identified 73 orthologous groups that responded to N starvation, demonstrated the existence of conserved N stress coupling mechanism among plants. Additional analysis of transcription profiles of microRNAs revealed differential expression of miR399 and miR530 under N starvation, suggesting their potential roles in plant nutrient homeostasis.