Phorbol esters promote postsynaptic accumulation of Vesl‐1S/Homer‐1a protein
Phorbol esters promote postsynaptic accumulation of Vesl‐1S/Homer‐1a protein
复制标题
佛波酯促进 Vesl-1S/Homer-1a 蛋白的突触后积累
DOI：
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发表时间：
2001
影响因子：
3.4
通讯作者：
H. Sugiyama
中科院分区：
文献类型：
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作者：
Akihiko Kato;T. Fukuda;Y. Fukazawa;Y. Isojima;K. Fujitani;K. Inokuchi;H. Sugiyama
我们研究了磷酯对培养海马神经元中活性调节蛋白Vesl - 1S/Homer - 1a的数量和亚细胞分布的影响。在控制条件下，在整个神经元体中检测到主要的Vesl‐1S免疫反应性（IR）。phorbol酯、PMA和PDBu的泡液应用导致Vesl - 1S蛋白的数量增加，并促进了神经元体皮质区域Vesl - 1S IR的点状分布。使用抗突触素和抗Vesl - 1S抗体的免疫荧光观察和电镜观察显示，在PMA应用后，Vesl - 1S在突触后区域积累。高浓度外源钾的膜去极化也促进了Vesl‐1S IR的点状分布。这些结果表明，phorbol触发的级联反应导致突触后区域Vesl - 1S蛋白的积累，并表明这些phorbol效应可能与突触活动引起的效应相似。
We examined effects of phorbol esters on the amount and the subcellular distribution of the activity‐regulated protein Vesl‐1S/Homer‐1a in cultured hippocampal neurons. Major Vesl‐1S immunoreactivity (IR) was detected throughout neuronal somata under control conditions. Bath application of phorbol esters, PMA and PDBu resulted in the increase in the amount of Vesl‐1S proteins and promoted punctate distribution of Vesl‐1S IR at the cortical regions of the neuronal somata. Immunofluorescent observations using antisynaptophysin and anti‐Vesl‐1S antibodies, and electron microscopic observations, revealed that Vesl‐1S accumulated at postsynaptic regions following PMA application. Membrane depolarization with high concentrations of external potassium also promoted the punctate distribution of Vesl‐1S IR. These results demonstrate that phorbol‐triggered reaction cascades result in the accumulation of Vesl‐1S protein at postsynaptic regions, and suggest that these phorbol effects may mimic those caused by synaptic activities.
DOI：
10.1073/pnas.95.22.13278
发表时间：
1998-10-27
影响因子：
11.1
作者：
Kawasaki, H;Sprihtgett, GM;Graybiel, AM
通讯作者：
Graybiel, AM