SGLT1 sugar transporter/sensor is required for post-oral glucose appetition
SGLT1 sugar transporter/sensor is required for post-oral glucose appetition
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DOI：
10.1152/ajpregu.00432.2015
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发表时间：
2016-04-01
影响因子：
2.8
通讯作者：
Ackroff, Karen
中科院分区：
文献类型：
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作者：
Sclafani, Anthony;Koepsell, Hermann;Ackroff, Karen
最近的研究结果表明，肠钠-葡萄糖转运蛋白1（SGLT 1）葡萄糖转运蛋白和传感器介导，部分，胃内（IG）葡萄糖和非代谢的α-甲基-D-吡喃葡萄糖苷（MDG）输注小鼠的食欲刺激作用。在这里，我们使用SGLT 1敲除（KO）和C57 BL/6 J野生型（WT）小鼠研究了SGLT 1在糖调节中的作用。最初的实验表明，KO和WT小鼠维持在非常低的碳水化合物饮食显示正常的偏好糖精，这是用于调味条件刺激（CS）的解决方案。在实验2中，训练小鼠饮用与IG MDG输注配对的一种调味溶液（CS+）和与IG水输注配对的不同调味溶液（CS-）。与WT小鼠相反，KO小鼠在训练期间减少而不是增加CS+的摄入，并且在选择测试中没有偏好CS+而不是CS-。在实验3中，KO小鼠也减少了它们对与IG葡萄糖配对的CS+的摄入，并且在选择测试中避免了CS+，不像WT小鼠，其偏好CS+而不是CS-。在实验4中，与WT小鼠一样，KO小鼠偏好葡萄糖+糖精溶液而非糖精溶液。这些发现支持SGLT 1参与口服葡萄糖后和MDG调节。结果还表明，KO小鼠中的糖吸收不良对糖摄入具有抑制作用，但不会阻止它们对甜味的天然偏好。
Recent findings suggest that the intestinal sodium-glucose transporter 1 (SGLT1) glucose transporter and sensor mediates, in part, the appetite-stimulation actions of intragastric (IG) glucose and nonmetabolizable alpha-methyl-D-glucopyranoside (MDG) infusions in mice. Here, we investigated the role of SGLT1 in sugar conditioning using SGLT1 knockout (KO) and C57BL/6J wild-type (WT) mice. An initial experiment revealed that both KO and WT mice maintained on a very low-carbohydrate diet display normal preferences for saccharin, which was used in the flavored conditioned stimulus (CS) solutions. In experiment 2, mice were trained to drink one flavored solution (CS+) paired with an IG MDG infusion and a different flavored solution (CS-) paired with IG water infusion. In contrast to WT mice, KO mice decreased rather than increased the intake of the CS+ during training and failed to prefer the CS+ over the CS- in a choice test. In experiment 3, the KO mice also decreased their intake of a CS+ paired with IG glucose and avoided the CS+ in a choice test, unlike WT mice, which preferred the CS+ to CS-. In experiment 4, KO mice, like WT mice preferred a glucose + saccharin solution to a saccharin solution. These findings support the involvement of SGLT1 in post-oral glucose and MDG conditioning. The results also indicate that sugar malabsorption in KO mice has inhibitory effects on sugar intake but does not block their natural preference for sweet taste.