PEPTIDE-YY - A NEUROPEPTIDE IN THE GUT - IMMUNOCYTOCHEMICAL AND IMMUNOCHEMICAL EVIDENCE
PEPTIDE-YY - A NEUROPEPTIDE IN THE GUT - IMMUNOCYTOCHEMICAL AND IMMUNOCHEMICAL EVIDENCE
复制标题
DOI：
10.1016/0306-4522(93)90472-r
复制
发表时间：
1993-07-01
期刊：
影响因子：
3.3
通讯作者：
SUNDLER, F
中科院分区：
文献类型：
--
作者：
BOTTCHER, G;EKBLAD, E;SUNDLER, F
应用免疫细胞化学和放射免疫分析结合高效液相色谱法，对大鼠、猫、雪貂和猪上消化道神经元进行了YY肽免疫反应性检测。所用的两种肽YY抗血清不识别相关肽神经肽Y和胰多肽。在大鼠肽YY-免疫反应性神经纤维几乎被限制到胃平滑肌的小弯曲，他们是众多的。肽YY免疫反应阳性神经细胞体发生在小弯的Serendum表面的小神经节。在猫和雪貂肽YY-免疫反应神经纤维发生在圆形平滑肌层的胃的小和大的弯曲，也在小肠上部;这样的纤维也有许多在肌间神经节在这些地区。在猪中，它们很少，但与猫和雪貂的分布大致相同，只是它们在靠近食管胃交界处的厚肌束中数量相当多。在猫、雪貂和猪的上消化道肌间神经节沿着和大鼠胃的浆膜神经节内存在肽YY免疫反应性神经细胞体，表明至少有一些肽YY免疫反应性纤维起源于或靠近肠壁。双染色实验表明，几乎所有含有肽YY的神经元和神经纤维都与储存神经肽Y的神经元和神经纤维不同。肽YY免疫反应性内分泌细胞不仅在较低的肠道，但也在胃的四个物种的研究。在胃窦中，这些细胞数量众多，构成了含胃泌素细胞的亚群。放免分析显示，四种动物胃粘膜和上消化道平滑肌中均含有YY样肽。高效液相色谱法的结果表明，肽YY样物质在上消化道是不同的神经肽Y和胰多肽和正宗的肽YY相同，除了在胃窦粘膜中，只有一小部分的肽YY免疫反应性物质洗脱像正宗的肽YY。
Peptide YY immunoreactivity was detected in neuronal elements in the upper digestive tract of the rat, cat, ferret and pig by immunocytochemistry and radioimmunoassay combined with high-performance liquid chromatography. The two peptide YY antisera used do not recognize the related peptides neuropeptide Y and pancreatic polypeptide. In the rat peptide YY-immunoreactive nerve fibres were virtually restricted to the stomach smooth muscle of the minor curvature where they were numerous. Peptide YY-immunoreactive nerve cell bodies occurred in small ganglia on the serosal surface of the minor curvature. In the cat and ferret peptide YY-immunoreactive nerve fibres occurred in the circular smooth muscle layer of both the minor and major curvatures of the stomach and also in the upper small intestine; such fibres were numerous also in myenteric ganglia in these regions. In the pig, they were few but had roughly the same distribution as in the cat and ferret, except that they were quite numerous in thick muscle bundles close to the oesophagogastric junction. The presence of peptide YY-immunoreactive nerve cell bodies within the myenteric ganglia along the upper digestive tract of cat, ferret and pig, and in serosal ganglia of the rat stomach, indicates that at least some of the peptide YY-immunoreactive fibres demonstrated originate in or close to the gut wall. Double-staining experiments revealed that virtually all peptide YY-containing neurons and nerve fibres were distinct from those storing neuropeptide Y. Peptide YY-immunoreactive endocrine cells were encountered not only in the lower intestines but also in the stomach of the four species studied. In the antrum such cells were numerous and constituted a subpopulation of the gastrin-containing cells. In the oxyntic mucosa they were few and contained somatostatin.Radioimmunoassay revealed peptide YY-like peptides in gastric mucosa and smooth muscle from the upper digestive tract of all four species examined. The results of high-performance liquid chromatography suggest that the peptide YY-like material in the upper digestive tract is distinct from neuropeptide Y and pancreatic polypeptide and identical with authentic peptide YY except in the antral mucosa where only a small proportion of the peptide YY-immunoreactive material eluted like authentic peptide YY.