Osteoprogenitor Cells from Bone Marrow and Cortical Bone: Understanding How the Environment Affects Their Fate
Osteoprogenitor Cells from Bone Marrow and Cortical Bone: Understanding How the Environment Affects Their Fate
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DOI：
10.1089/scd.2014.0351
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发表时间：
2015-05-01
影响因子：
4
通讯作者：
Tasciotti, Ennio
中科院分区：
文献类型：
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作者：
Corradetti, Bruna;Taraballi, Francesca;Tasciotti, Ennio
骨骼是一个动态器官，骨骼祖细胞和造血细胞共享和竞争空间。已从骨髓(BM-MSC)和皮质骨碎片(CBF-MSC)中鉴定并获得了可能的间充质干细胞(MSC)。在这项研究中，我们证明，尽管细胞共享一个共同的祖先，但驻留组织的结构属性的差异会影响细胞的行为，并使它们对刺激做出不同的反应。与骨髓类似，骨骼的皮质部分包含一组独特的细胞，这些细胞对常见的MSC相关标记呈阳性染色。这些细胞表现出不同的多向分化能力、克隆形成能力和免疫抑制能力。特别是，与BM-MSC相比，CBF-MSC体积更大，早期增殖率较低，对成骨谱系有更大的承诺，结构性地产生一氧化氮作为骨重建的介质，更容易对促炎细胞因子做出反应。我们的数据表明，组织微环境的影响使CBF-MSC在开发新的骨修复策略方面具有优势。
Bone is a dynamic organ where skeletal progenitors and hematopoietic cells share and compete for space. Presumptive mesenchymal stem cells (MSC) have been identified and harvested from the bone marrow (BM-MSC) and cortical bone fragments (CBF-MSC). In this study, we demonstrate that despite the cells sharing a common ancestor, the differences in the structural properties of the resident tissues affect cell behavior and prime them to react differently to stimuli. Similarly to the bone marrow, the cortical portion of the bone contains a unique subset of cells that stains positively for the common MSC-associated markers. These cells display different multipotent differentiation capability, clonogenic expansion, and immunosuppressive potential. In particular, when compared with BM-MSC, CBF-MSC are bigger in size, show a lower proliferation rate at early passages, have a greater commitment toward the osteogenic lineage, constitutively produce nitric oxide as a mediator for bone remodeling, and more readily respond to proinflammatory cytokines. Our data suggest that the effect of the tissue's microenvironment makes the CBF-MSC a superior candidate in the development of new strategies for bone repair.