Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson's disease
Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson's disease
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DOI：
10.1186/s13041-019-0471-2
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发表时间：
2019-05-14
期刊：
影响因子：
3.6
通讯作者：
Sasaki, Hidenao
中科院分区：
文献类型：
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作者：
Uwatoko, Hisashi;Hama, Yuka;Sasaki, Hidenao
MicroRNA (miRNA) 是内源性小 (18-25nt)、单链、非编码 RNA，在转录后基因表达调控中发挥关键作用。生物体液和组织中 miRNA 的表达谱在各种疾病中发生变化。多系统萎缩症（MSA）和帕金森病（PD）都被归类为β-突触核蛋白病，并且通常呈现相似的临床表现。本研究旨在鉴定 PD 患者、MSA 患者和健康对照血浆样本中不同表达的 miRNA。我们使用微阵列分析来筛选这些患者中上调和下调的 miRNA，并通过逆转录定量聚合酶链反应 (RT-qPCR) 分析所识别的 miRNA 的相对定量表达水平。在 MSA-C、MSA-P 或 PD 患者与健康对照之间，hsa-miR-671-5p、hsa-miR-19b-3p 和 hsa-miR-24-3p 的表达水平存在显着差异。 MSA-P和PD中的hsa-miR-671-5p水平低于MSA-C组和对照组，PD中的hsa-miR-19b-3p水平高于其他组，PD中的hsa-miR-24-3p水平高于MSA-C组。 Hsa-miR-671-5p 是第一个被证明在血浆中 MSA-C 和 MSA-P 之间表达不同的 miRNA。有趣的是，hsa-miR-19b-3p和hsa-miR-24-3p的表达水平呈正相关，表明这些miRNA可能参与PD发病机制中的相同过程。我们的研究结果表明 hsa-miR-671-5p、hsa-miR-19b-3p 和 hsa-miR-24-3p 可能反映 PD 和 MSA 的病理生理学或症状。
MicroRNAs (miRNAs) are endogenous small (18-25nt), single-stranded, non-coding RNAs that play key roles in post-transcriptional gene expression regulation. The expression profiles of miRNAs in biofluids and tissues change in various diseases. Multiple system atrophy (MSA) and Parkinson's disease (PD) are both categorized as -synucleinopathies and often present with similar clinical manifestations. This study aimed to identify miRNAs that are differently expressed in plasma samples of PD patients, MSA patients, and healthy controls. We used microarray analysis to screen for miRNAs that are up- and down-regulated in these patients and analyzed the relative-quantitative expression levels of the identified miRNAs by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Hsa-miR-671-5p, hsa-miR-19b-3p, and hsa-miR-24-3p showed significantly different expression levels among patients with MSA-C, MSA-P, or PD, and healthy controls. Hsa-miR-671-5p levels were lower in the MSA-P and PD than the MSA-C and control groups, hsa-miR-19b-3p levels were higher in the PD than the other groups, and hsa-miR-24-3p levels were higher in the PD than the MSA-C group. Hsa-miR-671-5p was the first miRNA shown to be expressed differently between MSA-C and MSA-P in plasma. Interestingly, the expression levels of hsa-miR-19b-3p and hsa-miR-24-3p were positively correlated, indicating that these miRNAs may be involved in the same processes in PD pathogenesis. Our findings suggest that hsa-miR-671-5p, hsa-miR-19b-3p, and hsa-miR-24-3p may reflect the pathophysiology or symptoms of PD and MSA.