Adipose tissue: A vital in vivo role in mammary gland development but not differentiation
Adipose tissue: A vital in vivo role in mammary gland development but not differentiation
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DOI：
10.1002/dvdy.10065
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发表时间：
2002-04-01
影响因子：
2.5
通讯作者：
Green, J
中科院分区：
文献类型：
--
作者：
Couldrey, C;Moitra, J;Green, J
乳腺的发育和分化是通过关键的间质-上皮相互作用进行的。尽管许多研究试图了解这些复杂的相互作用，但很难证明脂肪组织在乳腺发育和功能中的重要作用。利用白色脂肪组织缺失的A-ZIP/F-1转基因小鼠，研究脂肪细胞在乳腺发育分化中的作用。在没有WAT的情况下，发育不全的乳腺形成原状，但不能正常生长和分支，导致少数短而严重膨胀的导管。然而，在妊娠期间，即使在没有脂肪细胞的情况下，也会发生大量的上皮细胞分裂和肺泡细胞形成，这表明脂肪组织不是乳腺分化所必需的。乳腺移植显示，这些转基因小鼠的上皮细胞在放置在正常的基质环境中时，具有正常生长和分化的潜力。这些实验清楚地表明，乳腺中脂肪细胞的缺乏会导致基质-上皮相互作用的中断，从而阻止正常的乳腺发育。然而，原始的上皮原基含有完全具有肺泡分化能力的乳腺干细胞。2002年出版，Wiley-Liss，Inc.Dagger
Development and differentiation of the mammary gland occurs by means of critical stromal-epithelial interactions. Although many studies have attempted to understand these complex interactions, it has been difficult to demonstrate the essential role of adipose tissue in the development and function of the mammary gland. By using the A-ZIP/F-1 transgenic mice lacking in white adipose tissue (WAT), we have studied the role of adipocytes in mammary gland development and differentiation. In the absence of WAT, rudimentary mammary anlagen form but are unable to grow and branch normally, resulting in a few, short, severely distended ducts. However, during pregnancy, a tremendous amount of epithelial cell division and alveolar cell formation occurs even in the absence of adipocytes, illustrating that adipose tissue is not required for mammary gland differentiation. Mammary gland transplantation revealed that epithelial cells from these transgenic mice possess the potential for normal growth and differentiation when placed into a normal stromal environment. These experiments clearly demonstrate that the absence of adipocytes in the mammary gland results in disruption of stromal-epithelial interactions that prevent normal mammary gland development. The rudimentary epithelial anlage, however, contain mammary stem cells, which are fully capable of alveolar differentiation. Published 2002 Wiley-Liss, Inc.dagger