Dynamic light scattering: a practical guide and applications in biomedical sciences.
Dynamic light scattering: a practical guide and applications in biomedical sciences.
复制标题
DOI：
10.1007/s12551-016-0218-6
复制
发表时间：
2016-12-01
影响因子：
--
通讯作者：
Patel, Trushar R
中科院分区：
文献类型：
--
作者：
Stetefeld, Jorg;McKenna, Sean A;Patel, Trushar R
动态光散射（DLS），也称为光子相关光谱（PCS），是研究溶液中大分子扩散行为的一种非常强大的工具。扩散系数以及由此计算的流体动力学半径取决于大分子的大小和形状。在这篇综述中，我们提供了证据的有用性DLS研究蛋白质，核酸，蛋白质-蛋白质或蛋白质-核酸制剂的复合物的同质性，以及研究蛋白质-小分子相互作用。此外，我们提供的DLS的应用程序作为一种补充方法，分析ultracenthegation研究和作为一种筛选工具，以验证解决方案散射模型，使用确定的流体动力学半径的例子。
Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a very powerful tool for studying the diffusion behaviour of macromolecules in solution. The diffusion coefficient, and hence the hydrodynamic radii calculated from it, depends on the size and shape of macromolecules. In this review, we provide evidence of the usefulness of DLS to study the homogeneity of proteins, nucleic acids, and complexes of protein-protein or protein-nucleic acid preparations, as well as to study protein-small molecule interactions. Further, we provide examples of DLS's application both as a complementary method to analytical ultracentrifugation studies and as a screening tool to validate solution scattering models using determined hydrodynamic radii.