Identification of a Novel Alkaliphilic Esterase Active at Low Temperatures by Screening a Metagenomic Library from Antarctic Desert Soil
Identification of a Novel Alkaliphilic Esterase Active at Low Temperatures by Screening a Metagenomic Library from Antarctic Desert Soil
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DOI：
10.1128/aem.02597-08
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发表时间：
2009-07-01
影响因子：
4.4
通讯作者：
Cowan, Donald
中科院分区：
文献类型：
--
作者：
Heath, Caroline;Hu, Xiao Ping;Cowan, Donald
通过对从南极荒漠土壤中获得的大肠杆菌元基因组文库进行功能筛选，鉴定出一种新的酯酶。该序列由297个氨基酸组成，与异种伯克霍尔德氏菌的一种可能的酯酶仅有29%的相似性。该酶在7至54摄氏度的温度范围内和碱性pH水平下都具有活性，是一种潜在的工业应用候选者。
A novel esterase was identified through functional screening of a metagenomic library in Escherichia coli obtained from Antarctic desert soil. The 297-amino-acid sequence had only low (< 29%) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7 to 54 degrees C and at alkaline pH levels and is a potential candidate for industrial application.