AN IN-VIVO MODEL TO COMPARE HUMAN-LEUKOCYTE INFILTRATION IN CARCINOMA XENOGRAFTS PRODUCING DIFFERENT CHEMOKINES
AN IN-VIVO MODEL TO COMPARE HUMAN-LEUKOCYTE INFILTRATION IN CARCINOMA XENOGRAFTS PRODUCING DIFFERENT CHEMOKINES
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DOI：
10.1002/ijc.2910620514
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发表时间：
1995-09-04
影响因子：
6.4
通讯作者：
COLOMBO, MP
中科院分区：
文献类型：
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作者：
MELANI, C;PUPA, SM;COLOMBO, MP
在这项研究中，我们测试了人类癌症表达的细胞因子的模式是否可以解释作为抗肿瘤免疫应答的一部分的白细胞亚群的不同体内募集。采用逆转录聚合酶链反应（RT-PCR）、免疫荧光和ELISA等方法对两种肿瘤细胞系SK-OV-3卵巢癌和CALU-3肺癌进行了细胞因子表达和体外分泌的分析。虽然两种细胞系均显示粒细胞集落刺激因子（G-CSF）、粒细胞巨噬细胞-CSF（GM-CSF）、M-CSF、白细胞介素（IL-）1 α和IL-8的组成型表达，但仅CALU-3细胞系表达IL-10、RANTES（活化调节，正常T细胞表达和分泌）和单核细胞活化蛋白（MCP）-1。免疫组化法检测MCP-1和IL-8的表达。为了分析肿瘤释放的细胞因子是否调节肿瘤浸润中的白细胞，我们研究了在SK-0 Y-3和CALU-3肿瘤异种移植物附近注射的人外周血幼仔白细胞的分布。虽然SK-0 V-3不能募集人白细胞，并且似乎几乎不被鼠CD 45（+）细胞浸润，但CALU-3似乎被人白细胞快速且大量浸润，这在18-24小时内诱导肿瘤坏死。
In this study we tested whether the pattern of cytokines expressed by human carcinomas could account for a different in vivo recruitment of leukocyte subpopulations as a part of the anti-tumor immune response. Two carcinoma cell lines, SK-OV-3 ovary carcinoma and CALU-3 lung carcinoma, were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), immunofluorescence and ELISA for the expression and in vitro production of cytokines with chemotactic, proinflammatory and growth-stimulating activity. Although both cell lines displayed a constitutive expression of granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage-CSF (GM-CSF), M-CSF, interleukin (IL-) 1 alpha and IL-8, only CALU-3 cell line expressed IL-10, RANTES (Regulated upon Activation, Normal T Expressed and Secreted) and monocyte-activating protein (MCP)-1. MCP-1 and IL-8 were detected by immunohistochemistry on sections from tumors xenografted in nude mice. To analyze whether the tumor-released cytokines modulate leukocytes in tumor infiltration, we studied the distribution of human peripheral brood leukocytes injected in the proximity of SK-OY-3 and of CALU-3 tumor xenografts. While SK-OV-3 was unable to recruit human leukocytes and appeared to be barely infiltrated by murine CD45(+) cells, CALU-3 appeared to be rapidly and heavily infiltrated by human leukocytes which induced tumor necrosis within 18-24 hr. (C) 1995 Wiley-Liss, Inc.