Molecular diversity of arbuscular mycorrhizal fungi and their distribution patterns related to host-plants and habitats in a hot and arid ecosystem, southwest China
Molecular diversity of arbuscular mycorrhizal fungi and their distribution patterns related to host-plants and habitats in a hot and arid ecosystem, southwest China
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西南湿热干旱生态系统丛枝菌根真菌分子多样性及其与寄主植物和生境的分布格局
DOI：
10.1111/j.1574-6941.2009.00815.x
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发表时间：
2010-03-01
影响因子：
4.2
通讯作者：
Zhao, Zhi-Wei
中科院分区：
文献类型：
--
作者：
Li, Ling-Fei;Li, Tao;Zhao, Zhi-Wei
对休闲地和未受干扰地的孔颖草、木豆和扭异茅根丛枝菌根真菌（AMF）群落进行了研究。扩增并克隆了根丛枝菌根真菌大亚基rDNA基因。共筛选出2353个克隆，其中428个克隆进行了测序。共获得393条AMF序列，划分为100个操作分类单位。系统发育分析表明，AMF序列分别属于球囊霉属（Glomus）、无梗孢霉属（Acaulospora）和盾孢霉属（Scutellospora），其中球囊霉属（Glomus）为优势属。在393个AMF序列中，81%是新的。同一种植物上AMF的多样性在UL高于FL，这从分子水平上有力地证实了土壤扰动降低了AMF的种群数量和物种丰富度。结果表明，不同寄主植物和不同生境的丛枝菌根真菌群落结构存在显著差异。同一生境内不同植物种AMF群落的相似性高于不同生境同一植物种AMF群落的相似性。分子证据支持我们以前的假设，基于形态学分析，AMF社区的影响更大的栖息地相比，主机的偏好。
The communities of arbuscular mycorrhizal fungi (AMF) colonizing the roots of Bothriochloa pertusa, Cajanus cajan and Heteropogon contortus in a fallow land (FL) and an undisturbed land (UL) were characterized. The large subunit rDNA genes of AMF from roots were amplified and cloned. A total of 2353 clones were screened by restriction fragment length polymorphism, and 428 clones were subsequently sequenced. A total of 393 AMF sequences, which were grouped into 100 operational taxonomic units, were obtained. Phylogenetic analysis revealed that the AMF sequences belonged to Glomus, Acaulospora and Scutellospora, and that Glomus was the dominant genus. Of the 393 AMF sequences, 81% were novel. The diversity of AMF colonizing the same plant species was higher in the UL than in the FL, which confirmed strongly from the molecular evidence that soil disturbance reduced AMF population and species richness. The results revealed that AMF communities were significantly different among host-plant species and between the two habitats. The similarity of AMF communities colonizing different plant species within a habitat was higher than that of the same plant species from different habitats. The molecular evidence supported our previous hypothesis based on morphological analyses that AMF communities were more influenced by habitats compared with host preference.