Bacillus cereus-induced food-borne outbreaks in France, 2007 to 2014: epidemiology and genetic characterisation
Bacillus cereus-induced food-borne outbreaks in France, 2007 to 2014: epidemiology and genetic characterisation
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DOI：
10.2807/1560-7917.es.2016.21.48.30413
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发表时间：
2016-12-01
期刊：
影响因子：
19
通讯作者：
Brisabois, A.
中科院分区：
文献类型：
--
作者：
Glasset, B.;Herbin, S.;Brisabois, A.
本研究的目的是从2007年至2014年期间法国发生的140起强证据食源性疫情（FBO）相关的564株独特的国家收集中鉴定和鉴定蜡样芽孢杆菌。淀粉类食品和蔬菜是最常见的食品运输工具; 911例人间病例中有747例发生在机构餐饮环境中。通过M13-PCR方法筛选出与74株FBO严格相关的149株FBO，并对其编码催吐素（cereulide）、催吐毒素（Nhe、Hbl、CytK 1和CytK 2）和溶血素（HlyII）的基因进行了检测，并对panC系统发育进行了分类。这将菌株聚类为12个遗传特征（GS），突出了每个菌株的毒力潜力。GS1（仅nhe基因）和GS 2（nhe、hbl和cytK 2）是最普遍的GS，可能对人类健康产生重大影响，因为它们分别存在于28%和31%的FBO中。我们的研究为B的表征提供了一个方便的分子方案。蜡状芽孢杆菌菌株负责FBO，以提高监测和调查的B。蜡样诱导的FBO，评估新出现的集群和菌株的多样性。
The aim of this study was to identify and characterise Bacillus cereus from a unique national collection of 564 strains associated with 140 strong-evidence food-borne outbreaks (FBOs) occurring in France during 2007 to 2014. Starchy food and vegetables were the most frequent food vehicles identified; 747 of 911 human cases occurred in institutional catering contexts. Incubation period was significantly shorter for emetic strains compared with diarrhoeal strains A sub-panel of 149 strains strictly associated to 74 FBOs and selected on Coliphage M13-PCR pattern, was studied for detection of the genes encoding cereulide, diarrhoeic toxins (Nhe, Hbl, CytK1 and CytK2) and haemolysin (HlyII), as well as panC phylogenetic classification. This clustered the strains into 12 genetic signatures (GSs) highlighting the virulence potential of each strain. GS1 (nhe genes only) and GS2 (nhe, hbl and cytK2), were the most prevalent GS and may have a large impact on human health as they were present in 28% and 31% of FBOs, respectively. Our study provides a convenient molecular scheme for characterisation of B. cereus strains responsible for FBOs in order to improve the monitoring and investigation of B. cereus-induced FBOs, assess emerging clusters and diversity of strains.