Progesterone stimulation by prostaglandin F2alpha involves the protein kinase C pathway in cultured bovine luteal cells
Progesterone stimulation by prostaglandin F2alpha involves the protein kinase C pathway in cultured bovine luteal cells
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DOI：
10.1262/jrd.44.79
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发表时间：
1998-02-01
影响因子：
1.8
通讯作者：
Skarzynski, Dariusz Jan
中科院分区：
文献类型：
--
作者：
Okuda, Kiyoshi;Uenoyama, Yoshihisa;Skarzynski, Dariusz Jan
介导前列腺素F2alpha （PGF2alpha）刺激牛黄体细胞分泌黄体酮的细胞内机制尚不清楚。在本研究中，我们利用细胞培养系统研究了PGF2alpha通过激活蛋白激酶C （PKC）来刺激孕酮分泌的假设。牛黄体中期细胞（发色周期的第8-12天）培养24小时，然后暴露于不同浓度的pgf2 α（10-8、10-7、10-6 M）或黄体生成素（LH; 100 ng/ml）中4、6或16小时。用最高剂量的pgf2 α (10-6 M)或LH处理的细胞在所有三次暴露时间内都导致黄体酮分泌增加。当PKC被促瘤酚酯，12-肉豆蔻酸13-醋酸酯（PMA; 10-6 M）下调后，PGF2alpha的刺激作用不再明显，而LH的反应则不受影响。这些结果支持了PGF2alpha的促黄体作用是通过激活细胞内PKC介导的假设。
The intracellular mechanism that mediates the stimulatory effect of prostaglandin F2alpha (PGF2alpha) on progesterone secretion by bovine luteal cells is not well understood. In the present study, we investigated to test the hypothesis that PGF2alpha stimulates progesterone secretion through activation of protein kinase C (PKC) by using a cell culture system. Bovine mid-luteal cells (Days 8-12 of the estrous cycle) were cultured for 24 h and then exposed to varying concentrations of PGF2alpha (10-8, 10-7, 10-6 M) or luteinizing hormone (LH; 100 ng/ml) for 4, 6, or 16 h. Treatment of the cells with the highest dose of PGF2alpha (10-6 M) or LH resulted in increases in progesterone secretion for all three exposure times. Following down-regulation of PKC with a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate (PMA; 10-6 M), the stimulatory effect of PGF2alpha was no longer evident, whereas the response to LH was unaffected. These results support the hypothesis that the luteotropic action of PGF2alpha is mediated by the activation of intracellular PKC.