BIOPHYSICAL AND MOLECULAR MECHANISMS OF SHAKER POTASSIUM CHANNEL INACTIVATION
BIOPHYSICAL AND MOLECULAR MECHANISMS OF SHAKER POTASSIUM CHANNEL INACTIVATION
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DOI：
10.1126/science.2122519
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发表时间：
1990-10-26
期刊：
影响因子：
56.9
通讯作者：
ALDRICH, RW
中科院分区：
文献类型：
--
作者：
HOSHI, T;ZAGOTTA, WN;ALDRICH, RW
由果蝇Shaker基因编码的钾离子通道在膜电位变得更正时迅速激活和关闭。定点突变和单通道膜片钳记录被用来探索的分子转变，在非洲爪蟾卵母细胞中表达的Shaker钾通道失活的基础。现在已经确定了在失活中具有重要作用的氨基末端附近的区域。结果表明，该区域形成了一个胞质结构域，与开放通道相互作用，导致失活的模型。
The potassium channels encoded by the Drosophila Shaker gene activate and inactivate rapidly when the membrane potential becomes more positive. Site-directed mutagenesis and single-channel patch-clamp recording were used to explore the molecular transitions that underlie inactivation in Shaker potassium channels expressed in Xenopus oocytes. A region near the amino terminus with an important role in inactivation has now been identified. The results suggest a model where this region forms a cytoplasmic domain that interacts with the open channel to cause inactivation.