INTRAVENTRICULAR MICRODIALYSIS - A NEW METHOD FOR DETERMINING MONOAMINE METABOLITE CONCENTRATIONS IN THE CEREBROSPINAL-FLUID OF FREELY MOVING RATS
INTRAVENTRICULAR MICRODIALYSIS - A NEW METHOD FOR DETERMINING MONOAMINE METABOLITE CONCENTRATIONS IN THE CEREBROSPINAL-FLUID OF FREELY MOVING RATS
复制标题
DOI：
10.1016/0165-0270(88)90171-9
复制
发表时间：
1988-07-01
影响因子：
3
通讯作者：
ROBINSON, TE
中科院分区：
文献类型：
--
作者：
BECKER, JB;ADAMS, F;ROBINSON, TE
用在体微透析法测定自由活动大鼠侧脑室脑脊液中单胺类代谢产物(二羟基苯乙酸(DOPAC)、高香草酸(HVA)和5-羟基吲哚乙酸(5-HIAA)的浓度。这些代谢物的基线浓度和多巴胺(DA)周转率(通过200 mg/kg丙磺舒后DA代谢物的累积估计)都在其他方法采集脑脊液时报道的范围内。一系列的初步研究表明，该方法可用于重复采集脑脊液，并对病变、药物或移植物引起的局部多巴胺能活动变化敏感。(1)单侧黑质6-羟基多巴胺(6-OHDA)损毁后，损毁侧脑脊液中DOPAC和HVA的浓度较对侧或非损毁侧显著降低。(2)在单侧6-OHDA损毁动物的左侧和右侧脑室同时取样时，氟哌啶醇可引起双侧脑脊液中DOPAC和HVA浓度的升高。有趣的是，氟哌啶醇引起的脑脊液中DA代谢物浓度的增加在单侧6-OHDA损伤的大鼠的完整纹状体附近比没有损伤的大鼠更大。(3)在侧脑室移植肾上腺髓质组织的动物中，脑脊液中DOPAC的浓度比移植前或对照移植的动物升高。
A new method is described to estimate the cerebrospinal fluid (CSF) concentrations of monoamine metabolites (dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA)) in the lateral ventricle of freely moving rats by use of in vivo microdialysis. Both the baseline concentrations of these metabolites and the rate of dopamine (DA) turnover (estimated by the accumulation of total DA metabolites after 200 mg/kg probenecid) were within the range reported when other methods were used to sample CSF. A series of preliminary studies were conducted to demonstrate that this method can be used to repeatedly sample CSF, and to show that the method is sensitive to local changes in dopaminergic activity induced by lesions, drugs or grafts. (1) Unilateral 6-hydroxydopamine (6-OHDA) lesions of the substantia nigra produced a significant decrease in the CSF concentrations of DOPAC and HVA ipsilateral to the lesion, relative to the contralateral side or to concentrations in animals without lesions. (2) When left and right lateral ventricles were sampled simultaneously in animals with a unilateral 6-OHDA lesion, haloperidol induced an increase in DOPAC and HVA concentrations in CSF on both sides of the brain. Interestingly, the haloperidol-induced increase in CSF concentrations of DA metabolites was greater adjacent to the intact striatum of rats with unilateral 6-OHDA lesions than in animals with no lesion. (3) Finally, in animals with adrenal medulla tissue grafted into the lateral ventricle there was an increase in the CSF concentration of DOPAC compared to pregraft values or to those of animals with control grafts.