Stimulation by glutamate receptors of arachidonic acid release depends on the Na+/Ca2+ exchanger in neuronal cells.
Stimulation by glutamate receptors of arachidonic acid release depends on the Na+/Ca2+ exchanger in neuronal cells.
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发表时间：
1993-06
影响因子：
3.6
通讯作者：
A. Dumuis;M. Sebben;L. Fagni;L. Prézeau;O. Manzoni;E. Cragoe;J. Bockaert
中科院分区：
文献类型：
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作者：
A. Dumuis;M. Sebben;L. Fagni;L. Prézeau;O. Manzoni;E. Cragoe;J. Bockaert
在纹状体神经元原代培养中，刺激n -甲基- d -天冬氨酸（NMDA）受体或(RS)- α -氨基-3-羟基-5-甲基-4-异氧唑丙酸（AMPA）受体和代谢性谷氨酸受体（mGluR）的联合激活（但不是单独激活）通过激活磷脂酶A2 （PLA2）强烈增加花生四烯酸（AA）的释放。去极化剂，如缬曲定，在刺激AA释放方面与NMDA一样有效。然而，通过电压敏感的Ca2+通道增加细胞内Ca2+浓度不会导致PLA2的显著刺激。锂离子是一种单价阳离子，不参与Na+/Ca2+交换活性，但可渗透到嗜离子性谷氨酸受体通道，可阻断由veratridine或AMPA + mGluR激动剂诱导的AA释放。它也减少了nmda诱导的AA释放，但程度较轻。在缬曲定、NMDA受体或AMPA受体加mGluR刺激后，Na+/Ca2+交换剂对PLA2激活的贡献通过使用Na+/Ca2+交换剂的选择性抑制剂得到证实。
In primary cultures of striatal neurons, stimulation of N-methyl-D-aspartic acid (NMDA) receptors or associative activation (but not separate activation) of (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors and metabotropic glutamate receptors (mGluR) strongly increased arachidonic acid (AA) release via activation of phospholipase A2 (PLA2). Depolarizing agents, such as veratridine, were as potent as NMDA in stimulating AA release. However, increasing the intracellular Ca2+ concentration via voltage-sensitive Ca2+ channels did not result in a significant stimulation of PLA2. Substitution of sodium by lithium, a monovalent cation that does not participate in the Na+/Ca2+ exchanger activity but permeates ionotropic glutamate receptor channels, blocked AA release induced by veratridine or AMPA plus mGluR agonists. It also reduced the NMDA-induced AA release, to a lesser extent. The contribution of the Na+/Ca2+ exchanger to the activation of PLA2 after veratridine, NMDA receptor, or AMPA receptor plus mGluR stimulation was confirmed by using a selective inhibitor of the Na+/Ca2+ exchanger.