The midgut V-ATPase subunit A gene is associated with toxicity to crystal 2Aa and crystal 1Ca-expressing transgenic rice in Chilo suppressalis
The midgut V-ATPase subunit A gene is associated with toxicity to crystal 2Aa and crystal 1Ca-expressing transgenic rice in Chilo suppressalis
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中肠V-ATP酶亚基A基因与表达二化螟晶体2Aa和晶体1Ca的转基因水稻的毒性相关
DOI：
10.1111/imb.12570
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发表时间：
2019
影响因子：
2.6
通讯作者：
Ma W
中科院分区：
文献类型：
--
作者：
Qiu L;Sun Y;Jiang Z;Yang P;Liu H;Zhou H;Wang X;Zhang W;Lin Y;Ma W
由苏云金芽孢杆菌（Bt）产生的杀虫晶体（Cry）蛋白对多种昆虫有毒。目前已培育出转Cry 1A、Cry 2A和Cry 1C毒素基因的水稻，这些毒素对水稻二化螟（Chilo suppressalis）具有致死作用。确定Cry毒素与易感宿主相互作用的潜在机制将提高我们对Cry蛋白毒理学和Bt作物长期功效的理解。在这项研究中，我们测试了V-ATPase亚基A参与Cry 1Ab/1Ac，Cry 2Aa和Cry 1Ca毒素在C. suppressalis中的作用的假设。全长V-ATP酶亚基A转录本最初从C. suppressalis幼虫中肠克隆，然后用于产生双链RNA（dsRNA）产生细菌。使用转基因水稻品系TT 51（Cry 1AbandCry 1Acfusion基因）、T2 A-1（Cry 2Aa）和T1 C-19（Cry 1Ca）与V-ATP酶亚基AdsRNA处理的二化螟幼虫进行的毒性测定显示，幼虫对T2 A-1和T1 C-19转基因水稻的敏感性显著降低，但对TT 51水稻的敏感性没有降低。这些结果表明，V-ATP酶亚基A在介导Cry 2Aa和Cry 1Ca对二化螟的毒性中起关键作用。这些发现将对未来抗性管理工具的开发产生重要影响。
Insecticidal crystal (Cry) proteins produced by the bacteriumBacillus thuringiensis(Bt) are toxic to a diverse range of insects. Transgenic rice expressing Cry1A, Cry2A and Cry1C toxins have been developed that are lethal toChilo suppressalis, a devastating insect pest of rice in China. Identifying the mechanisms underlying the interactions of Cry toxins with susceptible hosts will improve both our understanding of Cry protein toxicology and long‐term efficacy of Bt crops. In this study, we tested the hypothesis that V‐ATPase subunit A contributes to the action of Cry1Ab/1Ac, Cry2Aa and Cry1Ca toxins inC. suppressalis. The full‐lengthV‐ATPase subunit Atranscript was initially cloned from theC. suppressalislarval midgut and then used to generate double‐stranded RNA (dsRNA)‐producing bacteria. Toxicity assays using transgenic rice lines TT51 (Cry1AbandCry1Acfusion genes), T2A‐1 (Cry2Aa), and T1C‐19 (Cry1Ca) in conjunction withV‐ATPase subunit AdsRNA‐treatedC. suppressalislarvae revealed significantly reduced larval susceptibility to T2A‐1 and T1C‐19 transgenic rice, but not to TT51 rice. These results suggest that the V‐ATPase subunit A plays a crucial role in mediating Cry2Aa and Cry1Ca toxicity inC. suppressalis. These findings will have significant implications on the development of future resistance management tools.