Experimental infection of mice with tightly coiled spiral bacteria ("Candidatus Helicobacter suis") originating from the pig stomach
Experimental infection of mice with tightly coiled spiral bacteria ("Candidatus Helicobacter suis") originating from the pig stomach
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DOI：
10.1016/s0021-9975(03)00024-0
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发表时间：
2003-08-01
影响因子：
0.8
通讯作者：
Park, JH
中科院分区：
文献类型：
--
作者：
Park, JH;Hong, JJ;Park, JH
小鼠（n = 34）口服感染了紧密螺旋菌（TCSB）的猪胃匀浆。在接种后16周至108周成对死亡的小鼠中，TCSB无一例外地存在于粘膜表面、胃窝、细胞间隙、表面上皮细胞的细胞质和胃腺腔中。组织病理学上，淋巴细胞和浆细胞浸润从8周开始，随着感染的进展逐渐增加。从64周开始，在固有层和粘膜下层观察到大滤泡的形成，同时表面上皮细胞严重坏死。基底粘膜腺上皮细胞明显发育不良，并穿过基底膜侵入粘膜下层。Western blotting、ELISA和免疫组化方法证实TCSB与幽门螺杆菌具有共同的抗原性。374 bp的16S rRNA片段序列与猪螺旋杆菌（Candidatus Helicobacter suis） 16S rRNA基因同源性100%。TCSB对小鼠胃粘膜的实验性感染与慢性胃炎和发育不良病变密切相关。(C) 2003 Elsevier Ltd.版权所有。
Mice (n = 34) were inoculated orally with a gastric homogenate from a pig infected with tightly coiled spiral bacteria (TCSB). In mice killed in pairs at 16 intervals up to 108 weeks post-inoculation (pi), TCSB were invariably found, mainly in the mucosal surface, gastric pits, intercellular spaces, cytoplasm of surface epithelial cells, and lumina of gastric glands. Histopathologically, infiltration of lymphocytes and plasma cells was seen from 8 weeks pi onwards, gradually increasing as infection progressed. From 64 weeks pi onwards, the formation of large follicles was observed in the lamina propria and submucosa, together with severe necrosis of surface epithelial cells. Glandular epithelial cells in the fundic mucosa were markedly dysplastic and intruded through the basement membrane into the submucosal layer. Common antigenicity between TCSB and Helicobacter pylori was demonstrated by Western blotting, ELISA, and immunohistochemistry. The sequence of the 16S rDNA fragment of 374 bp showed 100% homology with the 16S rRNA gene of "Candidatus Helicobacter suis". Experimental infection of the gastric mucosa of mice with TCSB was closely associated with chronic gastritis and dysplastic lesions. (C) 2003 Elsevier Ltd. All rights reserved.