EPR-Based Approach for the Localization of Paramagnetic Metal Ions in Biomolecules
EPR-Based Approach for the Localization of Paramagnetic Metal Ions in Biomolecules
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DOI：
10.1002/anie.201410396
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发表时间：
2015-02-02
影响因子：
16.6
通讯作者：
Schiemann, Olav
中科院分区：
文献类型：
--
作者：
Abdullin, Dinar;Florin, Nicole;Schiemann, Olav
金属离子在蛋白质和寡核苷酸的催化和折叠中起着重要的作用。因此，它们在这种生物分子的三维折叠中的定位是理解结构-功能关系的一个重要目标。介绍了一种基于电子顺磁共振(EPR)的远程测量金属离子局域化的三边测量方法。在天青的Cu2+中心上对该方法进行了测试，并讨论了影响方法精密度的因素。
Metal ions play an important role in the catalysis and folding of proteins and oligonucleotides. Their localization within the three-dimensional fold of such biomolecules is therefore an important goal in understanding structure-function relationships. A trilateration approach for the localization of metal ions by means of long-range distance measurements based on electron paramagnetic resonance (EPR) is introduced. The approach is tested on the Cu2+ center of azurin, and factors affecting the precision of the method are discussed.