Degradation of bisphenol A by white rot fungi, Stereum hirsutum and Heterobasidium insulare, and reduction of its estrogenic activity.
Degradation of bisphenol A by white rot fungi, Stereum hirsutum and Heterobasidium insulare, and reduction of its estrogenic activity.
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白腐真菌、Stereum hirsutum 和 Heterobasidium insulare 降解双酚 A，并降低其雌激素活性。
DOI：
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发表时间：
2005
影响因子：
2
通讯作者：
I. Choi
中科院分区：
文献类型：
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作者：
Soo;Bon;J. Choi;D. Choi;B. An;E. Jeung;I. Choi
用两株降解木质素的担子菌Stereum hirsutom和Heterobasidium insulare在培养条件下降解双酚A(BPA)，并用MCF-7细胞增殖试验(E-Screen)和PS2基因表达分析检测降解产物的雌激素活性。毛链霉菌和海岛链霉菌都表现出对BPA 100ppm的高度抗性，在30℃培养8d内菌丝生长完全完成，两种真菌对BPA的完全降解(约99%)需要7~14d的时间。当BPA浓度为10(-5)M时，MCF-7细胞增殖活跃，但在含有猪链霉菌和猪链霉菌的BPA培养液中，细胞株的增殖受到显著抑制。PS2mRNA的表达也得到了类似的结果。与单独用双酚A处理相比，多毛链霉菌和胰岛素假单胞菌处理的BPA培养上清液中PS2mRNA表达水平降低1.5倍。
Two lignin-degrading basidiomycetes, Stereum hirsutum and Heterobasidium insulare, were used to degrade bisphenol A (BPA) in culture, and the estrogenic activity of the degradation products was examined using MCF-7 cell proliferation assays (E-screen) and analysis of pS2 mRNA expression in MCF 7 cells. Both S. hirsutum and H. insulare showed high resistance to BPA 100 ppm, and their mycelial growth was fully completed within 8 d of incubation at 30 degrees C. It took 7 to 14 d to achieve complete degradation (ca. 99%) of BPA by both fungi. MCF-7 cells proliferated actively at a BPA concentration of 10(-5) M. However, cell line proliferation was significantly inhibited when the cells were incubated in BPA culture media containing S. hirsutum and H. insulare. Similar results were obtained regarding pS2 mRNA expression. The pS2 mRNA expression levels decreased by 1.5-fold in supernatant from BPA treated with S. hirsutum and H. insulare compared with those treated with BPA alone.
电子屏幕测定作为识别雌激素的工具：雌激素环境污染物的更新。
影响因子：
10.4
作者：
通讯作者：
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影响因子：
11.2
作者：
L. Schwartz;F. Koerner;S. Edgerton;J. Sawicka;M. Rio;J. Bellocq;P. Chambon;A. Thor
通讯作者：
L. Schwartz;F. Koerner;S. Edgerton;J. Sawicka;M. Rio;J. Bellocq;P. Chambon;A. Thor