Natural Surfactant Combined with Beclomethasone Decreases Lung Inflammation in the Preterm Lamb
Natural Surfactant Combined with Beclomethasone Decreases Lung Inflammation in the Preterm Lamb
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DOI：
10.1159/000328928
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发表时间：
2011-01-01
期刊：
影响因子：
3.7
通讯作者：
Buonocore, Giuseppe
中科院分区：
文献类型：
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作者：
Dani, Carlo;Corsini, Iuri;Buonocore, Giuseppe
背景资料：天然表面活性剂联合倍氯米松降低呼吸窘迫综合征早产羔羊的肺氧化应激。目的：检验这是通过减少肺部炎症而发生的假设。方法：早产羔羊接受200 mg/kg天然表面活性剂或200 mg/kg天然表面活性剂联合400或800 μ g/kg倍氯米松。测定支气管吸出物中白细胞介素8（IL-8）和巨噬细胞移动抑制因子（MIF），并评估肺力学。结果如下：IL-8在所有组中均增加，但在表面活性剂加400和800 μ g/kg倍氯米松治疗组中增加较低。MIF在表面活性剂组降低，在表面活性剂加400 μ g/kg倍氯米松组无变化，在表面活性剂加800 μ g/kg倍氯米松组降低。表面活性剂加800 μ g/kg倍氯米松组的MIF浓度高于其他组。结论：在RDS动物模型中，天然表面活性剂与800 μ g/kg倍氯米松联合使用可有效减轻肺部炎症，从而解释了肺氧化应激的相关降低。用表面活性剂加800 μ g/kg倍氯米松处理的动物中MIF的增加可能是新生儿肺的重要成熟和保护因素。版权所有（C）2011 S. Karger AG，巴塞尔
Background: Natural surfactant combined with beclomethasone decreases pulmonary oxidative stress in preterm lambs with respiratory distress syndrome (RDS). Objectives: To test the hypothesis that this occurs through a decrease in pulmonary inflammation. Methods: Preterm lambs received 200 mg/kg of natural surfactant or 200 mg/kg of natural surfactant combined with 400 or 800 mu g/kg of beclomethasone. Interleukin 8 (IL-8) and macrophage migration inhibitory factor (MIF) were assayed in bronchial aspirate samples and lung mechanics were evaluated. Results: IL-8 increased in all the groups, but the increase was lower in the groups treated with surfactant plus 400 and 800 mu g/kg of beclomethasone. MIF decreased in the surfactant group, did not vary in the surfactant plus 400 mu g/kg beclomethasone group, and decreased in the surfactant plus 800 mu g/kg beclomethasone group. MIF concentration was higher in the surfactant plus 800 mu g/kg beclomethasone group than in the other groups. Conclusions: Natural surfactant combined with beclomethasone at 800 mu g/kg is effective in reducing lung inflammation in an animal model of RDS, thus explaining the associated decrease in lung oxidative stress. The increase in MIF in animals treated with surfactant plus 800 mu g/kg of beclomethasone might be an important maturative and protective factor for neonatal lungs. Copyright (C) 2011 S. Karger AG, Basel