Colocalization of noggin and bone morphogenetic protein-4 during fracture healing
Colocalization of noggin and bone morphogenetic protein-4 during fracture healing
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DOI：
10.1359/jbmr.2001.16.5.876
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发表时间：
2001-05-01
影响因子：
6.2
通讯作者：
Takaoka, K
中科院分区：
文献类型：
--
作者：
Yoshimura, Y;Nomura, S;Takaoka, K
骨折修复过程中骨痂形成的调控涉及生长因子及其受体的协同表达，本文介绍了骨形态发生蛋白(BMP)拮抗剂noggin基因在骨折修复过程中的时空表达。用Northern印迹和原位杂交方法检测noggin在成年小鼠骨折修复模型中的表达，并与BMP-4的表达模式进行比较。在骨折愈伤组织形成的早期阶段，noggin Messenger RNA(MRNAs)的表达水平仅仅增强。Noggin mRNA的定位与BMP-4相似，骨折后2天，noggin mRNA信号主要定位于骨折部位附近的骨膜衬里细胞和皮质内膜。骨折后5、10和21d，新形成的骨痂中的软骨细胞和成骨细胞中均检测到noggin mRNA，其定位方式与BMP-4的定位方式无明显差异，提示noggin/BMP-4的平衡可能是影响骨折愈合过程中骨痂形成的重要因素。
The regulation of callus formation during fracture repair involves the coordinate expression of growth factors and their receptors, This article describes the temporal and spatial expression of noggin gene, an antagonist to bone morphogenetic protein (BMP), during the fracture repair process. Noggin expression was examined by means of Northern blotting and in situ hybridization and compared with the expression pattern of BMP-4 in a model of fracture repair in adult mice. Expression levels of noggin messenger RNA (mRNA) mere enhanced in the early phase of fracture callus formation. The localization of the noggin mRNA was similar to that of BMP-4 mRNA, Distinct noggin mRNA signals were located predominantly in cells lining the periosteum and the cortical endosteum near the fracture site at 2 days after fracture. At 5, 10, and 21 days after fracture, noggin mRNA was detected in the chondrocytes and osteoblasts in the newly formed callus, The pattern of localization was indistinguishable from that of BMP-4, These results suggest that the noggin/BMP-4 balance could be an important factor in the regulation of callus formation during fracture healing.