Atypical prothrombin in purified preparations from dicoumarol-treated steers.
Atypical prothrombin in purified preparations from dicoumarol-treated steers.
复制标题
来自双香豆素处理的小牛肉的纯化制剂中的非典型凝血酶原。
DOI：
10.1016/0024-3205(72)90026-4
复制
发表时间：
1972
期刊：
影响因子：
--
通讯作者：
O. Malhotra
中科院分区：
文献类型：
--
作者：
O. Malhotra
一种从双香豆素处理过的牛中获得的凝血酶原制剂，从柠檬酸钡洗脱液中得到高度纯化，并通过几个严格的理化和免疫学标准显示单一组分，仅具有正常特定生物活性的55%。在pH 8.6的琼脂凝胶电泳法中，有钙离子存在(但不含钙离子)，在β和α1之间产生正常的凝血酶原蛋白，在α1和α2之间产生不典型的变异体。这两个品种在免疫学上是相同的。非典型物质在1 1 2小时内被激活(正常为6分钟)，其比活性接近正常的12%。它对柠檬酸的吸附能力有别于我们已经鉴定的另外两个双香豆素诱导的凝血酶原变异体。
A preparation of prothrombin obtained from dicoumarol-treated steers, having been highly purified from an eluate of Ba citrate, and showing a single component by several rigorous physicochemical and immunological criteria, possessed only 55% of normal specific biologic activity. Agar gel electrophoresis at pH 8.6 in the presence of Ca 2+(but not its absence) yielded two separate components: normal prothrombrin moving between β and α 1, and an atypical variant between α 1 and α 2. The normal was separable from atypical by successive adsorptions on BaSO 4 in oxalated saline. The varieties were immunologically identical. Atypical material activated in 1 1 2 hours (as contrasted to 6 minutes for normal), and its specific activity approximated 12% of normal. Adsorbability on Ba citrate distinguished it from two other dicoumarol-induced prothrombin variants we have identified.