Transcriptome Analysis and Genes Function Verification of Root Development of Paeonia suffruticosa under Sandy Loam Cultivation
Transcriptome Analysis and Genes Function Verification of Root Development of Paeonia suffruticosa under Sandy Loam Cultivation
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沙壤土栽培牡丹根系发育的转录组分析及基因功能验证
DOI：
10.32604/phyton.2022.023572
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发表时间：
2022
影响因子：
1.7
通讯作者：
Yuke Sun
中科院分区：
文献类型：
--
作者：
Yinglong Song;Wenqian Shang;Zheng Wang;Songlin He;Xinya Meng;Liyun Shi;Yuxiao Shen;Dan He;Xueyuan Lou;Yuke Sun
牡丹试管苗生根较差，限制了牡丹的大规模商业化生产。本研究在转录组测序的基础上，对桑迪栽培条件下牡丹根系形成不同阶段的差异表达基因及其相关代谢途径进行了鉴定。根据六个数据库（NR、NT、GO、KEGG、COG和Swiss-Prot），生成总共31.63Gb原始数据，并注释了120，188个单一基因（平均长度为911.98bp）。对未萌发根原基期、诱导根原基期和根形成期的分析分别检测到与133、132和133条代谢途径相关的8，232、6，907和10，687个差异表达基因。两个显著差异表达的基因（Unigene13430_All和CL10096.Contig1_All）与生长素途径相关。全长Unigene13430_All编码序列（843 bp）编码280个氨基酸，而全长CL10096.Contig1_All编码序列（1，470 bp）编码489个氨基酸。Unigene13430_All和CL10096.Contig1_All被鉴定为IAA基因家族成员，分别命名为PsIAA 27和PsARF 19。qRT-PCR分析和功能验证表明，PsARF 19和PsIAA 27在牡丹实生苗、扦插苗和嫁接苗中的表达差异显著。PsARF 19促进根的发育，可能是一个与牡丹根形成相关的调控基因; PsIAA 27抑制侧根的发育，可能参与调控根形成过程中对生长素的敏感性。本研究结果为进一步研究芍药根形成的机理奠定了基础。转录组数据将是一个很好的资源，研究人员有兴趣在表征生根相关的牡丹基因。
Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony. In this study, on the basis of transcriptome sequencing, differentially expressed genes and the associated metabolic pathways were identified in tree peony roots at different stages of root formation under sandy loam cultivation. A total of 31.63 Gb raw data were generated and 120,188 unigenes (mean length of 911.98 bp) were annotated according to six databases (NR, NT, GO, KEGG, COG, and Swiss-Prot). Analyses of the ungerminated root primordium period, induced root primordium period, and root formation period detected 8,232, 6,907, and 10,687 differentially expressed genes related to 133, 132, and 133 metabolic pathways, respectively. Two significantly differentially expressed genes (Unigene13430_All and CL10096.Contig1_All) were associated with the auxin pathway. The full-length Unigene13430_All coding sequence (843 bp) encoded 280 amino acids, whereas the full-length CL10096.Contig1_All coding sequence (1,470 bp) encoded 489 amino acids. Unigene13430_All and CL10096.Contig1_All were identified as IAA gene family members and were respectively named PsIAA27 and PsARF19. The qRT-PCR analysis and functional verification indicated that the expressions of PsARF19 and PsIAA27 in tree peony seedlings, cuttings and grafted seedlings were significant different. PsARF19 promoted root development, it might be a regulatory gene related to the formation of tree peony roots, while PsIAA27 inhibited lateral root development, and it might be involved in controlling auxin sensitivity during root formation. The results of this study may form the basis of future investigations on the mechanism mediating peony root formation. The transcriptome data will be an excellent resource for researchers interested in characterizing the rooting-related tree peony genes.