Development of an analytical chip for colorimetric detection of medium-chain aldehydes by reaction with pararosaniline in porous glass
Development of an analytical chip for colorimetric detection of medium-chain aldehydes by reaction with pararosaniline in porous glass
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多孔玻璃中副玫瑰苯胺反应比色检测中链醛分析芯片的研制
DOI：
10.1016/j.talanta.2023.124382
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发表时间：
2023
期刊：
影响因子：
6.1
通讯作者：
Abe Natsumi
中科院分区：
文献类型：
--
作者：
Maruo Yasuko Y.;Kawamura Naoto;Abe Natsumi
中链醛类是常见的人类呼吸酶，可在肺部疾病患者的呼吸中检测到。因此，测量人类呼吸中的中链醛气体可以提供与这些疾病的潜在存在相关的重要的、非侵入性的和诊断信息。在这项研究中，分析芯片的中链醛检测不受短链醛的干扰。该分析芯片由浸渍有副玫瑰苯胺和酸的多孔玻璃（即，乙酸与少量磷酸）。在暴露于中链醛之后，红色分析芯片变为紫色，并且在620 nm处观察到吸收峰。结果发现，在多孔玻璃中发生了不可逆反应，因此，分析芯片以累积的方式发挥作用。测定了分析芯片在620 nm处的吸光度变化与壬醛暴露浓度之间的线性关系。重要的是，所开发的分析芯片成功地检测到壬醛浓度为8 - 270 ppb，根据暴露24小时后620 nm处的吸光度变化计算。此外，使用分析芯片照片的R值的变化估计壬醛浓度。该方法适用于床旁呼吸分析。最后，分析芯片也被发现是积极的辛醛和癸醛的相对灵敏度为0.7相比，壬醛，它是不活跃的短链醛。
Medium-chain aldehydes are common human biogases that can be detected in the breath of patients with lung diseases. As such, the measurement of medium-chain aldehyde gases in human breath can provide significant, noninvasive, and diagnostic information related to the potential presence of such diseases. In this study, an analytical chip is developed for the detection of medium-chain aldehydes without interference from short-chain aldehydes. This analytical chip is composed of porous glass impregnated with pararosaniline and an acid (i.e., acetic acid with small amount of phosphoric acid). After exposure to medium-chain aldehydes, the red analytical chip became violet in color, and an absorption peak was observed at 620 nm. It was found that a non-reversible reaction occurred in the porous glass, therefore, the analytical chip functions in a cumulative manner. A linear relationship was determined between the absorbance change of the analytical chip at 620 nm and the nonanal exposure concentration. Importantly, the developed analytical chip successfully detected nonanal at concentrations of 8–270 ppb as calculated from the absorbance change at 620 nm after a 24 h exposure time. In addition, nonanal concentration was estimated using the change in the R value of the analytical chip photograph. This method is suitable for point-of-care breath analysis. Finally, the analytical chip was also found to be active toward octanal and decanal with a relative sensitivity of 0.7 compared to that of nonanal; it was not active toward short-chain aldehydes.