Quantum dots as strain- and metabolism-specific microbiological labels
Quantum dots as strain- and metabolism-specific microbiological labels
复制标题
DOI：
10.1128/aem.69.7.4205-4213.2003
复制
发表时间：
2003-07-01
影响因子：
4.4
通讯作者：
Nadeau, JL
中科院分区：
文献类型：
--
作者：
Kloepfer, JA;Mielke, RE;Nadeau, JL
生物共轭量子点（QD）作为多波长荧光标记物在芯片生物检测和真核细胞中显示出巨大的潜力。然而，这些光致发光纳米晶体在细菌中的应用以前没有报道过，它们的大尺寸（3至10 nm）使得它们是否抑制细菌对附着分子的识别以及它们是否能够穿过细菌细胞壁尚不清楚。在这里，我们描述了使用共轭的CdSe量子点的菌株和代谢特异性微生物标记在各种各样的细菌和真菌，我们的分析是面向使用受体的共轭生物分子是存在的，并在生物体的表面上活跃。虽然细胞表面分子，如糖蛋白，使良好的目标共轭量子点，内部标记是不一致的，并导致大的光谱位移相比，原来的荧光，这表明有破碎或溶解的量子点。透射电子显微镜的整个坐骑和薄切片证实，细菌能够提取镉和硒从量子点的方式依赖于量子点表面共轭。
Biologically conjugated quantum dots (QDs) have shown great promise as multiwavelength fluorescent labels for on-chip bioassays and eukaryotic cells. However, use of these photoluminescent nanocrystals in bacteria has not previously been reported, and their large size (3 to 10 nm) makes it unclear whether they inhibit bacterial recognition of attached molecules and whether they are able to pass through bacterial cell walls. Here we describe the use of conjugated CdSe QDs for strain- and metabolism-specific microbial labeling in a wide variety of bacteria and fungi, and our analysis was geared toward using receptors for a conjugated biomolecule that are present and active on the organism's surface. While cell surface molecules, such as glycoproteins, make excellent targets for conjugated QDs, internal labeling is inconsistent and leads to large spectral shifts compared with the original fluorescence, suggesting that there is breakup or dissolution of the QDs. Transmission electron microscopy of whole mounts and thin sections confirmed that bacteria are able to extract Cd and Se from QDs in a fashion dependent upon the QD surface conjugate.