Temporal Profile of Biofilm Formation, Gene Expression and Virulence Analysis in Candida albicans Strains
Temporal Profile of Biofilm Formation, Gene Expression and Virulence Analysis in Candida albicans Strains
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DOI：
10.1007/s11046-016-0088-2
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发表时间：
2017-04-01
期刊：
影响因子：
5.5
通讯作者：
Cardoso Jorge, Antonio Olavo
中科院分区：
文献类型：
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作者：
de Barros, Patricia Pimentel;Rossoni, Rodnei Dennis;Cardoso Jorge, Antonio Olavo
不同毒力的白念珠菌菌株的特性对于了解真菌毒力机制非常有用。本研究的目的是评估和比较大蜡螟生物膜的形成、ALS 1、ALS 3、HWP 1、BCR 1、EFG 1、TEC 1、SAP 5、PLB 2和LIP 9的基因表达以及毒力的时间分布。白色念珠菌ATCC 18804和从HIV阳性患者（CA 60）分离的临床样品。尽管ATCC菌株体外形成的生物膜中CFU/mL计数较高，但C.与ATCC菌株相比，白色念珠菌菌株的Ca 60升高，尤其是在基因HWP 1、ALS 3、SAP 5、PLB 2和LIP 9（上调）中。Ca 60对G.在存活测定（p = 0.0394）和血细胞密度（p = 0.0349）中，与编码菌丝表达和Ca 60水解酶基因的上调基因一致。综上所述，C.本研究中使用的白色念珠菌菌株在生物膜形成量、体内毒力和所分析的基因的转录谱方面存在差异，这些基因可以改变与念珠菌定植、增殖和存活相关的因素。不同壁龛的白色念珠菌。SAP 5和HWP 1是在体外生物膜形成过程中表达较多的基因。
The characterization of Candida albicans strains with different degrees of virulence became very useful to understand the mechanisms of fungal virulence. Then, the objective of this study was to assess and compare the temporal profiles of biofilms formation, gene expression of ALS1, ALS3, HWP1, BCR1, EFG1, TEC1, SAP5, PLB2 and LIP9 and virulence in Galleria mellonella of C. albicans ATCC18804 and a clinical sample isolated from an HIV-positive patient (CA60). Although the CFU/mL counting was higher in biofilms formed in vitro by ATCC strain, the temporal profile of the analysis of the transcripts of the C. albicans strains was elevated to Ca60 compared to strain ATCC, especially in the genes HWP1, ALS3, SAP5, PLB2 and LIP9 (up regulation). Ca60 was more pathogenic for G. mellonella in the survival assay (p = 0.0394) and hemocytes density (p = 0.0349), agreeing with upregulated genes that encode the expression of hyphae and hydrolase genes of Ca60. In conclusion, the C. albicans strains used in this study differ in the amount of biofilm formation, virulence in vivo and transcriptional profiles of genes analyzed that can change factors associated with colonization, proliferation and survival of C. albicans at different niches. SAP5 and HWP1 were the genes more expressed in the formation of biofilm in vitro.