Treatment of spinal tuberculosis in rabbits using bovine serum albumin nanoparticles loaded with isoniazid and rifampicin
Treatment of spinal tuberculosis in rabbits using bovine serum albumin nanoparticles loaded with isoniazid and rifampicin
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DOI：
10.1080/01616412.2021.1979749
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发表时间：
2021-09-29
影响因子：
1.9
通讯作者：
Ge, Zhaohui
中科院分区：
文献类型：
--
作者：
Ma, Rong;Zhang, Jianqun;Ge, Zhaohui
目的评价异烟肼利福平牛血清白蛋白纳米粒（INH-RFP-BSA-NPs）治疗家兔脊柱结核的临床疗效。方法将35只家兔脊柱结核模型分为三组，A、B组各14只，C组7只。A组全部采用INH-RFP-BSA-NPs注射液治疗，B组采用INH、RFP经典剂型治疗，C组给予生理盐水作为空白对照。干预后，分别于第6周和第12周对所有兔子进行体重和CT扫描，以及血液和组织中INH和RFP浓度的测量。结果A组家兔在治疗6周和12周内体重分别增加0.44 kg和0.27 kg。注射后6周在病灶椎体内测定INH 1.64μg中心点g(-1)和RFP中心点g(-1)21.36μg的杀菌浓度，六周后椎体中INH和RFP的浓度仍维持在1.96μg中心点g(-1)和22.35μg中心点的水平分别为g(-1)。治疗12周后，CT扫描显示所有坏死组织均被正常骨组织取代。 B组所有兔子体重均无明显增加，4只兔子出现后腿瘫痪。椎体和病灶中INH和RFP的浓度远低于A组。治疗12周后CT扫描显示病灶椎体仅部分修复。结论 INH-RFP-BSA-NPs具有体内缓释、病灶椎体内靶向生物分布的特点。其对家兔脊柱结核的治疗效果远优于普通INH和RFP。
Objective To evaluate the clinical efficacy of bovine serum albumin nanoparticles loaded with isoniazid and rifampicin (INH-RFP-BSA-NPs) in the treatment of spinal tuberculosis in rabbits. Methods 35 spinal tuberculosis rabbit models were grouped into three groups, including 14 in group A and group B respectively and 7 in group C.All rabbits in group A were treated by INH-RFP-BSA-NPs's injection and in group B were treated with classic dosage form of INH and RFP, while in group C normal saline was given as the blank control. After intervention, the body weighing and CT scan, as well as concentration's measurement of INH and RFP in blood and tissues, were performed in all rabbits at the time of the 6thweek and 12th week, respectively. Results In group A, rabbits' weight increased by 0.44 kg and 0.27 kg within 6 weeks and 12 weeks' treatment respectively. The bactericidal concentrations of 1.64 mu g center dot g(-1) for INH and 21.36 mu g center dot g(-1) for RFP were measured in focus vertebral body 6 weeks post-injection and six weeks later the concentrations of INH and RFP in vertebral body still maintained at the level of 1.96 mu g center dot g(-1) and 22.35 mu g center dot g(-1)respectively. After 12 weeks therapy, CT-scanned showed all the necrotic tissue was replaced by normal bone tissue. In group B, all rabbits had no significant increment of body weight and 4 rabbits had paralysis of hind leg. The concentrations of INH and RFP in vertebral body and focus were much lower than group A. CT-scanned showed the focus vertebral body was only partially repaired after 12 weeks' therapy. Conclusion The INH-RFP-BSA-NPs has the characteristics of sustained release in vivo and target biodistribution in focus vertebral body. Its therapeutic effect in rabbit spinal tuberculosis is much better than common INH and RFP.