The brain tumor window model: a combined cranial window and implanted glioma model for evaluating intraoperative contrast agents.
The brain tumor window model: a combined cranial window and implanted glioma model for evaluating intraoperative contrast agents.
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DOI：
10.1227/01.neu.0000367631.02903.50
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发表时间：
2010-04
期刊：
影响因子：
4.8
通讯作者：
Sagher O
中科院分区：
文献类型：
--
作者：
Orringer DA;Chen T;Huang DL;Armstead WM;Hoff BA;Koo YE;Keep RF;Philbert MA;Kopelman R;Sagher O
用于脑肿瘤描绘的光学造影剂先前已在来自植入神经胶质瘤的动物的离体标本中进行了评价，并且可能不能反映手术期间遇到的真实视觉参数。本研究描述了一种新的模型系统，旨在评估光学造影剂在体内肿瘤勾画。对8周龄Sprague-Dawley大鼠进行双顶颅骨切除术。脑实质内注射9 L胶质瘤细胞。用氰基丙烯酸酯胶将盖玻片粘合到颅骨缺损处。当肿瘤半径达到1 mm时，静脉注射考马斯亮蓝，同时记录皮质表面的外观。使用红/绿色/蓝色成分的计算机图像分析来量化肿瘤和非肿瘤组织之间的可见差异，并将脑肿瘤窗（BTW）模型中的描绘与常规9 L神经胶质瘤模型进行比较。BTW模型中的肿瘤边缘在造影剂给药前定义不清楚，但在造影剂给药后很明显。基于红色分量强度，BTW模型中在造影剂施用后50分钟肿瘤描绘改善了4倍（P<0.002）。在相同剂量考马斯亮蓝下，常规9 L胶质瘤模型对胶质瘤的勾画程度高于BTW模型（P<0.03）。在植入的胶质瘤上放置窗口在技术上是可行的，并且在大鼠中耐受性良好。BTW模型是一个有效的系统，用于评估光学造影剂设计描绘脑肿瘤边缘。据我们所知，我们已经描述了第一个在体内模型系统，用于评估光学造影剂的肿瘤划定。
Optical contrast agents for brain tumor delineation have been previously evaluated in ex vivo specimens from animals with implanted gliomas and may not reflect the true visual parameters encountered during surgery. This study describes a novel model system designed to evaluate optical contrast agents for tumor delineation in vivo. Biparietal craniectomies were performed on 8-week-old Sprague-Dawley rats. 9L glioma cells were injected intraparenchymally. A cover slip was bonded to the cranial defect with cyanoacrylate glue. When the tumor radius reached 1mm, coomassie blue was administered intravenously while the appearance of the cortical surface was recorded. Computerized image analysis of the red/green/blue color components was used to quantify visible differences between tumor and non-neoplastic tissue and to compare delineation in the brain tumor window (BTW) model to the conventional 9L glioma model. The tumor margin in the BTW model was poorly defined prior to contrast administration, but readily apparent post-contrast administration. Based on red component intensity, tumor delineation improved four-fold at 50 minutes post-contrast administration in the BTW model (P<0.002). The conventional 9L glioma model overestimated the degree of delineation compared to the BTW model at the same dose of coomassie blue (P<0.03). Window placement overlying an implanted glioma is technically possible and well tolerated in the rat. The BTW model is a valid system for evaluating optical contrast agents designed to delineate brain tumor margins. To our knowledge, we have described the first in vivo model system for evaluating optical contrast agents for tumor delineation.