CIRCADIAN REGULATION OF BIOLUMINESCENCE IN GONYAULAX INVOLVES TRANSLATIONAL CONTROL
CIRCADIAN REGULATION OF BIOLUMINESCENCE IN GONYAULAX INVOLVES TRANSLATIONAL CONTROL
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DOI：
10.1073/pnas.86.1.172
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发表时间：
1989-01-01
影响因子：
11.1
通讯作者：
HASTINGS, JW
中科院分区：
文献类型：
--
作者：
MORSE, D;MILOS, PM;HASTINGS, JW
本文报道了海洋沟鞭藻Gonyaulax polyedra中的LBP含量的10倍昼夜变化。这种蛋白质结合并稳定生物发光底物-。在夜晚早期，当生物发光增加并且细胞中LBP水平上升时，脉冲标记实验表明LBP在体内快速合成。在其他时候，LBP的合成速率至少低50倍，而大多数其他蛋白质的合成速率保持不变。LBP的mRNA水平，在体外翻译和RNA（北方）杂交测定，不改变在每天的周期，表明在这个物种的生物发光的昼夜节律控制是由翻译介导的。
A 10-fold circadian variation in the amount of luciferin binding protein (LBP) in the marine dinoflagellate Gonyaulax polyedra is reported. This protein binds and stabilizes luciferin, the bioluminescence substrate. In early night phase, when bioluminescence is increasing and LBP levels are rising in the cell, pulse labeling experiments show that LBP is being rapidly synthesized in vivo. At other times, the rate of LBP synthesis is at least 50 times lower, while the rate of synthesis of most other proteins remains the same. The LBP mRNA levels, as determined by in vitro translations and by RNA (Northern) hybridizations, do not vary over the daily cycle, indicating that circadian control of bioluminescence in this species is mediated by translation.