Effects of Synthetic Diets Enriched in Specific Nutrients on Drosophila Development, Body Fat, and Lifespan.
Effects of Synthetic Diets Enriched in Specific Nutrients on Drosophila Development, Body Fat, and Lifespan.
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DOI：
10.1371/journal.pone.0146758
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Reis T
中科院分区：
文献类型：
--
作者：
Reis T
基因与饮食的相互作用在肥胖易感性中发挥着至关重要但人们知之甚少的作用。因此，开发遗传易处理的模型系统来研究饮食对肥胖遗传背景的影响是当前研究的焦点。在这里，我提出了一种改良的合成果蝇饮食，针对幼虫的及时发育进行了优化，这是一个专门用于能量储存的阶段。特别是增加单个常量营养素（碳水化合物、脂质或蛋白质）的水平会产生显着不同的机体效应。高碳水化合物饮食会对发育时间、体型、早期寿命和身体脂肪产生不利影响。引人注目的是，将膳食脂质含量增加四倍却没有这些影响。富含蛋白质的饮食似乎是最有益的，因为幼虫发育得更快，而且体型没有变化，成为长寿的成虫。我相信这种合成饮食将极大地促进有机体能量平衡中基因-饮食相互作用的研究。
Gene-diet interactions play a crucial but poorly understood role in susceptibility to obesity. Accordingly, the development of genetically tractable model systems to study the influence of diets in obesity-prone genetic backgrounds is a focus of current research. Here I present a modified synthetic Drosophila diet optimized for timely larval development, a stage dedicated to energy storage. Specifically increasing the levels of individual macronutrients–carbohydrate, lipid, or protein–resulted in markedly different organismal effects. A high-carbohydrate diet adversely affected the timing of development, size, early lifespan and body fat. Strikingly, quadrupling the amount of dietary lipids had none of these effects. Diets rich in protein appeared to be the most beneficial, as larvae developed faster, with no change in size, into long-lived adults. I believe this synthetic diet will significantly facilitate the study of gene-diet interactions in organismal energy balance.