Evidence of systemic dissemination of Chlamydia pneumoniae via macrophages in the mouse
Evidence of systemic dissemination of Chlamydia pneumoniae via macrophages in the mouse
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DOI：
10.1086/515280
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发表时间：
1998-05-01
影响因子：
6.4
通讯作者：
Campbell, LA
中科院分区：
文献类型：
--
作者：
Moazed, TC;Kuo, CC;Campbell, LA
据推测，肺炎衣原体通过感染单核细胞/巨噬细胞并通过血液或淋巴管传播而引起全身性疾病。为了研究肺炎衣原体如何传播，确定了该生物体在体内感染小鼠巨噬细胞的能力以及感染是否可以通过巨噬细胞转移。通过直接铺板、分离和聚合酶链反应在鼻内接种小鼠的肺泡巨噬细胞和腹膜内接种小鼠的腹膜巨噬细胞中检测肺炎衣原体。在鼻内和腹膜内接种的小鼠的外周血单核细胞中也检测到肺炎衣原体，但在血浆中未检测到。当通过腹腔注射将肺泡或腹膜巨噬细胞从感染小鼠体内过继转移至未感染小鼠时，通过聚合酶链反应在肺、胸腺、脾脏和/或腹部淋巴结中检测到肺炎衣原体DNA。这些结果证明了肺炎衣原体感染体内巨噬细胞并通过受感染的巨噬细胞通过血行和淋巴途径进行全身传播的能力。
Chlamydia pneumoniae has been postulated to cause systemic disease by infection of monocytes/macrophages and spread via the blood or lymphatics. To investigate how C. pneumoniae disseminates, the ability of the organism to infect murine macrophages in vivo and whether infection can be transferred via macrophages were determined. C. pneumoniae was detected by direct plating, isolation, and polymerase chain reaction in alveolar macrophages from intranasally inoculated mice and peritoneal macrophages from intraperitoneally inoculated mice. C. pneumoniae were also detected in peripheral blood mononuclear cells, but not plasma, of intranasally and intraperitoneally inoculated mice. When alveolar or peritoneal macrophages were adoptively transferred by intraperitoneal injection from infected to uninfected mice, C. pneumoniae DNA was detected by polymerase chain reaction in lung, thymus, spleen, and/or abdominal lymph nodes. These results demonstrate the ability of C. pneumoniae to infect macrophages in vivo and to disseminate systemically via infected macrophages by hematogenous and lymphatic routes.