Construction and Characterization of a Novel Superantigen Fusion Protein: bFGF/SEB
Construction and Characterization of a Novel Superantigen Fusion Protein: bFGF/SEB
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DOI：
10.1080/07357900802487228
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发表时间：
2009-01-01
影响因子：
2.4
通讯作者：
Xiaoming-Yang
中科院分区：
文献类型：
--
作者：
Lichan-Wang;Huajie-Zhang;Xiaoming-Yang
背景资料：葡萄球菌肠毒素（Staphylococcal enterotoxins，SE）作为细菌超抗原之一，是T细胞，特别是表达T细胞受体V链的T细胞的有效激活剂，可诱导IFN-、TNF-、IL-1、IL-2、IL-6、IL-12等细胞因子的产生，与特异性识别肿瘤细胞的载体协同作用，可用于肿瘤靶向治疗。材料与方法：扩增了葡萄球菌肠毒素B（SE B）的编码序列，并与人碱性成纤维细胞生长因子（bFGF）融合。重组蛋白SEB及bFGF/SEB融合蛋白的表达和纯化。检测其体外对脾细胞增殖、细胞因子产生及肿瘤细胞杀伤活性。此外，还采用免疫荧光技术检测了bFGF/SEB与肿瘤细胞的结合及肿瘤细胞的凋亡。结果如下：与天然SEA和重组SEB相比，融合蛋白bFGF/SEB具有相似的生物学活性，包括抑瘤率。结论：重组bFGF/SEB融合蛋白保留了SEB的超抗原活性，有望成为一种新型的肿瘤免疫治疗剂。
Background: As one of the bacterial superantigens, Staphylococcal enterotoxins (SEs) are potent activators of T cells, especially for those expressing T cell receptor V chains, and can induce the production of cytokines such as IFN-, TNF-, IL-1, IL-2, IL-6, IL-12, etc. Thus, SEs could be used in tumor-targeting therapy when cooperated with the vectors that can specifically recognize the tumor cells. Materials and methods: The coding sequences of Staphylococcal enterotxin B (SEB) was amplified and fused with human basic fibroblast growth factor (bFGF). Recombinant protein SEB and fusion protein bFGF/SEB were expressed and purified. The biological activity was detected, including splenocytes proliferation, cytokine production, and cytotoxicity in tumor cells in vitro. In addition, the binding of bFGF/SEB with tumor cells and the tumor cell apoptosis were also tested by immunofluorescent technique. Results: The fusion protein bFGF/SEB had similar biological activities compared with natural SEA and recombinant SEB, including tumor-inhibition ratio. Conclusion: The recombinant bFGF/SEB-fusion protein was shown to retain the superantigenic activity of SEB, and might be a novel promising immunotherapeutic agent for the treatment of some carcinomas.