Anticardiolipin wet workshop report. Fifth International Symposium on antiphospholipid antibodies.
Anticardiolipin wet workshop report. Fifth International Symposium on antiphospholipid antibodies.
复制标题
抗心磷脂湿法车间报告。
DOI：
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发表时间：
1994
影响因子：
3.5
通讯作者：
David Birch
中科院分区：
文献类型：
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作者：
E. Harris;S. Pierangeli;David Birch
第五届国际抗磷脂研讨会（1992年9月9-12日，德克萨斯州圣安东尼奥）举办了一个抗心磷脂研讨会。讲习班有四个目标：1。确定8种未知血清中免疫球蛋白（Ig） G、IgM和IgA抗心磷脂测量的变异性；2. 检验三种商用酶联免疫吸附测定（ELISA）试剂盒与抗磷脂标准化实验室之间的相关性；3. 检查抗心磷脂与磷脂抗原和β 2糖蛋白1 （β 2GP1）结合的比较方案；和4。来检测β 2GP1对心磷脂结合活性的影响。结果显示，车间参与者和抗磷脂标准化实验室之间的抗心磷脂定量测量结果具有良好的总体相关性。与单个ELISA试剂盒的相关性也很好。关于第三个目标，所有参与者都显示出与心磷脂和磷脂酰丝氨酸的可比性结合，但没有与磷脂酰胆碱或β 2GP1的结合。最后，β 2GP1增强了抗心磷脂结合，但不是必需的。本次研讨会不会解决关于抗心磷脂测量和特异性的争议，但会提出解决这些问题的方案。
An anticardiolipin wet workshop was conducted at the Fifth International Antiphospholipid Symposium (San Antonio, Texas, September 9-12, 1992). The workshop had four objectives: 1. to determine variability in measurement of immunoglobulin (Ig) G, IgM, and IgA anticardiolipin of eight unknown sera; 2. to examine the correlation between three commercial enzyme-linked immunosorbent assay (ELISA) kits and the Antiphospholipid Standardization Laboratory; 3. to examine protocols by which anticardiolipin binding to phospholipid antigens and beta 2 glycoprotein 1 (beta 2GP1) might be compared; and 4. to examine the beta 2GP1 effect on cardiolipin binding activity. Results showed good overall correlation of quantitative anticardiolipin measurements between workshop participants and the Antiphospholipid Standardization Laboratory. Correlation with individual ELISA kits was also good. With respect to the third objective, all participants showed comparable binding to cardiolipin and phosphatidylserine but none to phosphatidylcholine or beta 2GP1. Finally, beta 2GP1 enhanced, but was not essential for, anticardiolipin binding. This workshop will not settle controversies about anticardiolipin measurement and specificity, but suggests protocols to resolve these issues.