Ploidy variability in greenhouse cultured and in vitro propagated potato (Solanum tuberosum) monohaploids (2n=x=12) as determined by flow cytometry
Ploidy variability in greenhouse cultured and in vitro propagated potato (Solanum tuberosum) monohaploids (2n=x=12) as determined by flow cytometry
复制标题
通过流式细胞术测定温室培养和体外繁殖的马铃薯（Solanum tuberosum）单单倍体（2n=x=12）的倍性变异
DOI：
10.1007/bf00268493
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发表时间：
1987
影响因子：
6.2
通讯作者：
B. Uijtewaal
中科院分区：
文献类型：
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作者：
B. Uijtewaal
用流式细胞术研究了23个马铃薯单单倍体无性系的DNA分布。所有单单倍体无性系在DNA分布上存在差异，但叶片中没有单倍体细胞。在2 ~ 3年的单倍体水平上均高度稳定。对不同因素对叶片细胞DNA分布影响的研究表明，离体茎尖繁殖材料的多倍体细胞比例低于温室栽培植物。在原生质体分离过程中，体外培养的植物材料的酶处理导致DNA分布向低c值方向显著转移，而机械纯化步骤导致单倍体核的选择性丢失。可以检测到季节对DNA模式的影响。
The DNA distributions of 23 different monohaploid potato clones were investigated by flow-cytometric measurements. All monohaploid clones differed in DNA distribution but none of them contained only monoploid cells in the leaves. All were highly stable on the monohaploid level for 2–3 years. Investigation of the influence of different factors on the DNA distribution in leaf cells showed that the material derived from in vitro shoot tip propagation contained a lower proportion of polyploidized cells than greenhouse grown plants. With protoplast isolation the enzyme treatment of in vitro cultured plant material induced a striking shift of DNA distribution towards the lower C-value whereas the mechanical purification steps caused a selective loss of monoploid nuclei. Seasonal influence on the DNA patterns could be detected.