Evaluation of a Commercially Available Hyaluronic Acid Hydrogel (Restylane) as Injectable Scaffold for Dental Pulp Regeneration: An In Vitro Evaluation
Evaluation of a Commercially Available Hyaluronic Acid Hydrogel (Restylane) as Injectable Scaffold for Dental Pulp Regeneration: An In Vitro Evaluation
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DOI：
10.1016/j.joen.2016.10.026
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发表时间：
2017-02-01
影响因子：
4.2
通讯作者：
Diogenes, Anibal
中科院分区：
文献类型：
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作者：
Chrepa, Vanessa;Austab, Obadab;Diogenes, Anibal
简介：再生牙髓手术（REP）是治疗未成熟牙齿的可行替代方案，但这些手术并不能预见地导致牙髓牙本质再生。真正的牙髓再生生物工程方法需要结合有利于牙髓-牙本质复合物再生的支架。已经提出了几种材料作为 REP 的支架；然而，大多数人不符合立即在临床椅旁使用的资格。因此，本研究的目的是评估 Restylane（一种经美国食品和药物管理局批准的透明质酸基凝胶）作为 REP 的可能支架。方法： 根尖乳头 (SCAP) 干细胞单独培养或与 Restylane 或 Matrigel 支架混合培养。将各组在基础培养基中培养6、24和72小时，并评估细胞活力。对于矿化分化实验，各组在分化培养基中培养 7 天并进行碱性磷酸酶活性处理，或者培养 14 天并通过使用定量逆转录聚合酶链式反应进行基因表达处理。基础培养基中的SCAP作为对照。结果：与对照相比，Restylane 或 Matrigel 中的细胞封装显示细胞活力降低。尽管如此，与对照组相比，Restylane 组的细胞活力在 3 天的过程中显着增加，而 Matrigel 组的细胞活力显着下降。与对照组相比，Restylane 显着提高了碱性磷酸酶活性，并上调了牙本质唾液酸磷蛋白、牙本质基质酸性磷蛋白-1 和基质细胞外磷酸糖蛋白。结论：美国食品和药物管理局批准的基于透明质酸的注射凝胶可促进 SCAP 存活、矿化和分化为 SCAP。成牙本质细胞表型，可能是一种有前途的 REP 支架材料。
Introduction: Regenerative endodontic procedures (REPs) are viable alternatives for treating immature teeth, yet these procedures do not predictably lead to pulp-dentin regeneration. A true bioengineering approach for dental pulp regeneration requires the incorporation of a scaffold conducive with the regeneration of the pulp-dentin complex. Several materials have been proposed as scaffolds for REPs; nonetheless, the majority are not eligible for immediate clinical chairside use. Thus, the aim of this study was to evaluate Restylane, a Food and Drug Administration-approved hyaluronic acid based gel, as possible scaffold for REPs. Methods: Stem cells of the apical papilla (SCAP) were cultured either alone or in mixtures with either Restylane or Matrigel scaffolds. Groups were cultured in basal culture medium for 6, 24, and 72 hours, and cell viability was assessed. For the mineralizing differentiation experiments, groups were cultured in differentiation medium either for 7 days and processed for alkaline phosphatase activity or for 14 days and processed for gene expression by using quantitative reverse-transcription polymerase chain reaction. SCAP in basal medium served as control. Results: Cell encapsulation in either Restylane or Matrigel demonstrated reduced cell viability compared with control. Nonetheless, cell viability significantly increased in the Restylane group in the course of 3 days, whereas it decreased significantly" in the Matrigel group. Restylane promoted significantly greater alkaline phosphatase activity and upregulation of dentin sialophosphoprotein, dentin matrix acidic phosphoprotein-1, and matrix extracellular phosphoglycoprotein, compared with control. Conclusions: A Food and Drug Administration-approved hyaluronic acid based injectable gel promoted SCAP survival, mineralization, and differentiation into an odontoblastic phenotype and may be a promising scaffold material for REPs.