Mesonia sediminis sp. nov., isolated from a sea cucumber culture pond
Mesonia sediminis sp. nov., isolated from a sea cucumber culture pond
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DOI：
10.1007/s10482-015-0575-3
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发表时间：
2015-09
期刊：
影响因子：
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通讯作者：
Fengqing Wang;zhi-hong xie;Jin-Xin Zhao;Guanjun Chen;Z. Du
中科院分区：
文献类型：
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作者：
Fengqing Wang;zhi-hong xie;Jin-Xin Zhao;Guanjun Chen;Z. Du
从中国荣成（122°14′34″ E36 °54′36″N）海参养殖池的底泥中分离到一株产黄色、革兰氏阴性、兼性厌氧细菌MF 326 T。MF 326 T菌株的过氧化氢酶呈阴性，氧化酶呈阳性。发现最佳生长发生在30 °C和pH 7.0-7.5，在2.0- 3.0%（w/v）NaCl存在下。系统发育分析表明，MF 326 T菌株与模式菌株Mesonia ostrae的同源性为94.3%，其次为Mesonia algae的同源性为93.9%。菌株MF 326 T的优势脂肪酸被鉴定为iso-C15：0，一种当量链长为13.565和antiso-C15：0的未知脂肪酸。发现主要极性脂质是两种未鉴定的脂质和磷脂酰乙醇胺。发现主要呼吸醌是MK-6，基因组DNA G+C含量测定为40.7mol%。根据系统发育分析和表型差异特征，认为菌株MF 326 T（=KCTC 42255 T =MCCC 1H 00125 T）应归属于Mesonia属，为一新种Mesonia sediminissp的模式菌株。十一是建议。
A yellow-pigmented, Gram-stain negative and facultatively anaerobic bacterium, designated MF326T, was isolated from a sample of sediment collected from a sea cucumber culture pond in Rongcheng, China (122°14′34″E 36°54′36″N). Cells of strain MF326Twere found to be catalase negative and oxidase positive. Optimal growth was found to occur at 30 °C and pH 7.0–7.5 in the presence of 2.0–3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MF326Tis a member of the genusMesoniaand exhibits the high sequence similarity (94.3 %) with the type strain ofMesonia ostreae, followed byMesonia algae(93.9 %). The dominant fatty acids of strain MF326Twere identified as iso-C15:0, an unidentified fatty acid with an equivalent chain-length of 13.565 and anteiso-C15:0. The major polar lipids were found to be two unidentified lipids and phosphatidylethanolamine. The major respiratory quinone was found to be MK-6 and the genomic DNA G+C content was determined to be 40.7 mol%. On the basis of the phylogenetic analysis and differential phenotypic characteristics, it is concluded that strain MF326T(=KCTC 42255T=MCCC 1H00125T) should be assigned to the genusMesoniaas the type strain of a novel species, for which the nameMesonia sediminissp. nov. is proposed.